中文摘要：本研究旨在探索饲料中添加群体猝灭（QQ）的本土益生菌，对亚洲鲈鱼的生长、消化酶活性、血液-生化响应、肠道菌群以及抗哈维氏弧菌的影响。本研究开展为期6周的实验，对亚洲花鲈分别饲喂基础饲粮 (对照组)和含有FU苏云金芽孢杆菌QQ1或蜡样芽孢杆菌QQ2的基础饲粮。研究发现，饲喂添加QQ微生物制剂的饲粮35 d后（感染前期），生长参数及消化酶活性均发生了显著变化(p < 0.05)；QQ1显著提高(p < 0.05)了鱼的血细胞比容(Hct)和总白细胞计数(TLC)；益生菌组的呼吸爆发活力(RBA)显著升高(p < 0.05)，鱼的血清总胆固醇水平显著降低(p < 0.05)。感染哈维氏弧菌后(第42天)，QQ1和QQ2中Hct和RBA、QQ2中球蛋白和QQ1中TLC显著提高(p < 0.05)；感染后益生菌治疗组的血清甘油三酯、胆固醇、碱性磷酸酶(ALP)、丙氨酸转氨酶(ALAT)、天冬氨酸转氨酶(ASAT)和乳酸脱氢酶(LDH)均显著低于(p < 0.05)PC组；QQ益生菌显著提高(p < 0.05)了亚洲鲈鱼对哈维氏弧菌的累积存活率。综上所述，蜡样芽孢杆菌QQ2，特别是苏云金芽孢杆菌QQ1在提高亚洲鲈鱼生产性能方面具有较大的潜力，可以有效地用于哈维氏弧菌的防治。
外文摘要：The use of probiotics is a promising and increasingly common strategy to maintain a healthy farming environment in an eco-friendly and sustainable manner. The goal of this study was to investigate the possible effects of dietary supplementation with autochthonous quorum quenching (QQ) probiotics on growth, digestive enzymes activity, haemato-biochemical responses, gut microbial communities and resistance against Vibrio harveyi in Asian seabass. Asian seabass (75 +/- 0.6 g and 15.5 +/- 0.3 cm) were fed with basal diet (control groups) and basal diet containing 1 x 10(9) CFU per g Bacillus thuringiensis QQ1 or Bacillus cereus QQ2 for 6 weeks. Feeding with diets supplemented with QQ probiotics for 35 days (pre-infection period) resulted in a significant (p < 0.05) alteration of the growth parameters (RGR, FCR, SGR, PER, FER and FI) and digestive enzymes activity (alpha-amylase, total protease, lipase, trypsin, alkaline phosphatase, T/C and A/T). Hematocrit (Hct) and total leucocyte count (TLC) were significantly (p < 0.05) elevated in fish fed with QQ1. Respiratory burst activity (RBA) likewise significantly (p < 0.05) increased in the probiotic groups relative to the control groups. Moreover, a significant decrease (p < 0.05) in the level of serum total cholesterol was observed in fish fed with probiotic. QQ1 and QQ2 were able to colonize the host's intestine at high number after 5 weeks. Hct and RBA in QQ1 and QQ2, globulin in QQ2 and TLC in QQ1 were significantly (p < 0.05) promoted compared to the positive control (PC) after infection with Vibrio harveyi (day 42). Serum triglycerides, cholesterol, alkaline phosphatase (ALP), alanine aminotransferase (ALAT), aspartate aminotransferase (ASAT) and lactate dehydrogenase (LDH) were significantly (p < 0.05) lower in the probiotic treatments than the PC group after infection. QQ probionts significantly (p < 0.05) improved the cumulative survival of Asian seabass against V. harveyi. According to these findings, we suggest that B. cereus QQ2 and particularly B. thuringiensis QQ1 have a high potential to enhance the performances of Asian seabass and therefore can be effectively applied for the control of V. harveyi.
外文关键词：Quorum quenching probiotic; Bacillus; Performance; Survival; Asian seabass; Vibrio harveyi
作者：Ghanei-Motlagh, R；Mohammadian, T；Gharibi, D；Khosravi, M；Mahmoudi, E；Zarea, M；El-Matbouli, M；Menanteau-Ledouble, S
作者单位：Univ Vet Med；Shahid Chamran Univ Ahvaz；Islamic Azad Univ
期刊名称：AQUACULTURE
期刊影响因子：3.225
出版年份：2021
出版刊次：531
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