中文摘要：白头翁汤（BTW）为中药方剂，几百年来一直用于治疗溃疡性结肠炎（UC）。本研究探讨了BTW和肠道菌群对葡聚糖硫酸钠(DSS)诱导的UC小鼠的消炎作用，并研究了BTW初步治疗UC的作用机理。本研究旨在通过分子生物学和高通量测序来阐明BTW治疗UC的机制。研究发现，BTW能有效减轻UC小鼠的症状和组织病理学评分，减少IL-6、IL-1 β和tnf - α的产生；BTW治疗也抑制了IL-6/STAT3通路的激活；此外，16s rDNA测序显示，DSS组小鼠肠道菌群较对照组紊乱。经BTW治疗，肠道菌群多样性显著提高；在门水平上，厚壁菌门、拟杆菌门、变形菌门的比例降低；在属水平上，大肠杆菌-志贺菌的相对丰度降低，乳酸杆菌和阿克曼氏菌的相对丰度增加。由此可见，BTW能明显改善急性UC小鼠的炎症症状，潜在作用机制可能与多种信号通路有关，包括调节肠道菌群和炎症信号通路等。
外文摘要：Ethnopharmacological relevance: Baitouweng (BTW) decoction, a Chinese traditional medicine prescription, has been used to treat ulcerative colitis (UC) over hundreds of years. In this study, we investigated the anti-inflammatory effects of BTW and intestinal flora of dextran sulfate sodium (DSS)-induced UC mice, and we investigated the mechanism of BTW in the preliminary treatment of UC.
   Aim of study: The aim of this study was to elucidate the mechanism of BTW in treating UC through molecular biology and high-throughput sequencing.
   Methods: DSS-induced UC mice were established and randomly divided into the following four groups: control group, DSS group, BTW group and sulfasalazine (SASP) group. Except for the control group, 3% DSS drinking water was given to each group for 7 days, and the other two groups were intragastrically administered with BTW and SASP. Mice were sacrificed after gavage for 10 days. Body weight loss, disease activity index (DAI), colon length, colon histopathology and the expression of inflammatory cytokines were measured. Intestinal content samples were collected, and intestinal flora differences were analyzed by 16 S rDNA sequencing.
  Results: BTW effectively reduced the symptoms and histopathological score of UC mice, and it reduced the production of IL-6, IL-1 beta and TNF-alpha. Activation of the IL-6/STAT3 pathway was also suppressed by BTW treatment. Moreover, 16 S rDNA sequencing showed that the intestinal flora of mice in the DSS group was disordered compared to the control group. After treatment with BTW, the diversity of intestinal flora was significantly improved. At the phylum level, the proportion of Firmicutes to Bacteroidetes was decreased, and the ratio of Proteobacteria was decreased. At the genus level, the relative abundance of Escherichia-Shigella was decreased, but that of Lactobacillus and Akkermansia were increased.
   Conclusion: BTW significantly improved the inflammatory symptoms of mice with acute colitis, and the latent mechanism of BTW may be related to various signaling pathways, including the modulation of intestinal microflora and inflammatory signaling pathways, such as IL-6/STAT3.
 
外文关键词：Baitouweng decoction；Ulcerative colitis；Dextran sulfate sodium；Intestinal flora；IL-6/STAT3 pathway
作者：Xuan-qing, CHEN；Xiang-yu, LV；Shi-jia, LIU
作者单位：Nanjing Univ Chinese Med；China Pharmaceut Univ
期刊名称：JOURNAL OF ETHNOPHARMACOLOGY
期刊影响因子：3.69
出版年份：2021
出版刊次：265
点击下载：白头翁汤通过调节肠道微生物和IL-6/STAT3信号通路减轻葡聚糖硫酸钠诱导的溃疡性结肠炎