中文摘要：本研究评估了饲粮中添加丁酸梭菌对鲤鱼肠道酶活性、肠道组织形态、免疫相关基因表达、短链脂肪酸和肠道微生物的影响。选取360尾鲤鱼，分为3组，分别饲喂添加0、0.125%和0.5%丁酸梭菌的饲料（用NC、LCB和HCB表示）8周。研究发现，添加丁酸梭菌对生长性能无显著影响。HCB组肠内过氧化氢酶(CAT)和溶菌酶(LZM)活性显著提高，丁酸和丙酸含量在两组中均升高。HCB组黏膜粘蛋白分泌明显增加，上皮微绒毛高度明显增加，黏膜皱褶更深更平滑。HCB组肠内IL-10、TLR-2、MyD-88、ZO-1、Occludin基因表达显著升高，而LCB组无明显差异。饲粮中添加丁酸梭菌可提高鲤鱼肠道微生物多样性。在门水平上，拟杆菌门的丰度提高，梭杆菌门和变形杆菌门的丰度显著降低。在属水平上，HCB组有益细菌Bacteroides和Cetobacterium的相对丰度显著增加，而气单胞菌、弧菌和假单胞菌等病原体受到抑制。可见，饲粮中添加适量的丁酸梭菌可通过调节肠道微生物群，改善鲤鱼肠道屏障防御功能，从而提高鲤鱼肠道免疫力。
外文摘要：This study evaluated the effects of dietary Clostridium butyricum on intestinal enzyme activity, intestinal histology, immune-related gene expression, short-chain fatty acids and intestinal microbiota in common carp (Cyprinus carpio L.). Three hundred and sixty fish (89.89 +/- 0.77 g) were divided into three groups and fed a diet supplemented with C. butyricum at 0, 0.125% (0.25 x 10(7) CFU/g), or 0.5% (1 x 10(7) CFU/g) (denoted as NC, LCB and HCB, respectively) for 8 weeks. Compared with the NC group, growth performances were not affected by adding C. butyricum. The enzyme activity of catalase (CAT) and lysozyme (LZM) in the intestine of the HCB group were significantly enhanced, and the butyric acid and propionic acid content in both C. butyricum-treated groups increased. In the HCB group, mucin secretion and the height of the epithelium microvilli were obviously increased, and the mucosal folds were deeper and smoother. The intestinal gene expression of IL-10, TLR-2, MyD-88, ZO-1 and Occludin were significantly higher in the HCB group than that in the control group but not in the LCB group. Dietary C. butyricum increased the diversity of the intestinal microbiota of common carp. At the phylum level, the abundance of Bacteroidetes improved concomitantly with a significant decrease in the abundance of Fusobacteria and Proteobacteria. At the genus level, the relative abundance of the beneficial bacteria Bacteroides and Cetobacterium increased remarkably in the HCB group, and pathogens such as Aeromonas, Vibrio, and Pseudomonas were inhibited. In conclusion, the results from this study indicate that supplementing the diet with C. butyricum at an appropriate dose can improve the intestinal immunity of common carp by regulating the intestinal microbiota and improving the defence function of the intestinal barrier.
外文关键词：Clostridium butyricum；Cyprinus carpio L.；Intestinal microbiota；Immunity
作者：Meng, XL；Wu, SK；Hu, WP；Zhu, ZX；Yang, GK；Zhang, YM；Qin, CB；Yang, LP；Nie, GX
作者单位：Henan Normal Univ
期刊名称：AQUACULTURE
期刊影响因子：3.225
出版年份：2021
出版刊次：530
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