中文摘要：从中国传统食品红曲米中成功地分离出紫红曲霉M-32，将其发酵产物以不同浓度（T0：0g/kg；T1：5g/kg；T2：10g /kg；T3：20g /kg）添加到凡纳滨对虾的饲粮中。8周后，T1和T2组的FCR显著降低，T3组的SR显著提高；T2组肝胰淀粉酶（AMS）活性显著提升。研究发现，M-32发酵产物显著提高了GSH活性，降低了MDA水平；显著提高了对虾的AKP和LYZ活性；显著改善了肠道绒毛高度、绒毛宽度、肌肉厚度等形态学结构；可调节对虾肠道微生物组成，显著提高乳酸菌的相对丰度。T1、T2和T3组的肠道菌群与对照组存在显著差异。此外，添加紫红曲霉发酵产物增强了对虾对副溶血性弧菌的抗性。综上所述，使用紫红曲霉M-32菌株作为饲料添加剂，对凡纳滨对虾的生长性能、免疫应答、肠道健康和抗病能力均有促进作用。
外文摘要：Monascus purpureus M-32 was successfully isolated from the Chinese traditional food red mould rice, and its fermentation product was supplemented in the diet of Litopenaeus vannamei at different concentrations (T0: 0 g/ kg; T1: 5 g/kg; T2: 10 g/kg and T3: 20 g/kg). After 8 weeks, the dietary administration of the M-32 fermentation product significantly decreased the FCR in groups T1 and T2 and significantly increased the SR in group T3 compared to those in the control (T0) (P < 0.05). The hepatopancreatic amylase (AMS) activity of group T2 was significantly higher than that of the control group (P < 0.05). The antioxidative activity was evaluated by determining GSH-Px, SOD and T-AOC activities and MDA levels, and the results indicated that supplementation with the M-32 fermentation product significantly improved GSH activity and reduced the MDA level (P < 0.05). The analysis of immune parameters showed that the activities of AKP and LYZ increased significantly in shrimp fed the M-32 fermentation product (P < 0.05). Intestinal histological observations demonstrated that M-32 could significantly improve the intestinal morphological structure, including measures such as villus height, villus width, and muscular thickness (P < 0.05). Microbiota analysis showed that the M-32 fermentation product modulated the intestinal microbial composition, and the relative abundance of Lactobacillus increased significantly in shrimp fed with M. purpureus. Principal coordinate analysis (PCoA) and non-metric multi-dimensional scaling (NMDS) analysis showed that the gut microbiota of groups T1, T2 and T3 was significantly different from that of the control group. Additionally, the supplementation of the M. purpureus fermentation product enhanced the resistance of the shrimp against V. parahaemolyticus infection. In conclusion, the use of M. purpureus strain M-32 as a feed additive was beneficial to L. vannamei by promoting growth performance, the immune response, intestinal health and disease resistance.
外文关键词：Monascus purpureus；Fermentation product；Intestinal microbiota；Litopenaeus vannamei；Growth；Immune response
作者：Wang, P；Chen, SQ；Wei, CY；Yan, QP；Sun, YZ；Yi, GF；Li, D；Fu, WL
作者单位：Jimei Univ；Fujian Dabeinong Aquat Sci & Tech Co Ltd
期刊名称：AQUACULTURE
期刊影响因子：3.225
出版年份：2021
出版刊次：530
点击下载：紫红曲霉M-32改善凡纳滨对虾的生长性能、免疫应答、肠道形态、微生物群和抗病能力