中文摘要：致敏真菌孢子对个人和公共健康安全有潜在的负面影响，因此快速、灵敏地检测这些孢子成为了目前研究的热点。基于真菌孢子和特异性结合肽修饰的金纳米粒子(AuNPs)之间的相互作用，本研究提出了一种简单、快速地检测黑曲霉的比色法。通过噬菌体展示筛选鉴定出黑曲霉孢子结合肽配体，该配体通过特异性的多价效应，表现出比普通空气细菌更强的亲和力。将肽配体固定在AuNPs上，快速与黑曲霉孢子结合，使孢子沉淀后上清液的颜色强度发生明显变化。结合智能手机的图像分析应用程序，该简单比色法具有约50个孢子的高灵敏度，检测时间<10分钟。利用纳米金配体的特异性和纳米金的光学特性，该方法可简单快速地检测规模较大的目标微生物。
外文摘要：The rapid and sensitive detection of allergenic fungal spores is of great interest owing to the potentially negative impact of these spores on personal and public health and safety. We present a simple and prompt colorimetric strategy for detecting Aspergillus niger based on interactions between fungal spores and gold nanoparticles (AuNPs) modified with a specific binding peptide. The A. niger spore-binding peptide ligand was identified by phage display screening, which exhibited enhanced affinity through multivalent effects with specificity over common airborne bacteria. Immobilization of peptide ligands on AuNPs enabled rapid binding to A. niger spores, resulting in a visible change in the color intensity of the supernatant after sedimentation of the spores. This simple colorimetric approach displayed a high sensitivity of similar to 50 spores and a rapid detection time of <10 min when used with a smartphone-based image analysis application. This strategy can be adapted for the simple and rapid detection of target microorganisms of relatively large size by exploiting the specificity of ligands and the optical properties of gold nanoparticles.
外文关键词：Fungi sensor；Aspergillus niger spore；Peptide ligand；Gold
作者：Lee, JI；Jang, SC；Chung, J；Choi, WK；Hong, C；Ahn, GR；Kim, SH；Lee, BY；Chung, WJ
作者单位：Sungkyunkwan Univ；Dankook Univ；Korea Univ
期刊名称：SENSORS AND ACTUATORS B-CHEMICAL
期刊影响因子：7.1
出版年份：2021
出版刊次：327
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