中文摘要：选用32头青春期前期的母猪，分别饲喂基础日粮(CON)和添加了300g/d膳食纤维(fibre)、240 g/d豆油(SO)或两者同时添加(fibre+SO)的CON日粮，研究饲粮中添加膳食纤维对饲喂高脂日粮的猪卵泡闭锁的影响。在第4个发情周期的第19天，日粮中添加SO的母猪，闭锁卵泡增加了112%；凋亡标记物、Bax和半胱氨酸天冬氨酸蛋白酶3的表达增加，而这些影响在饲喂膳食纤维后减轻。饲喂纤维可增加血清和卵泡液中血清素和褪黑素的浓度。总之，膳食纤维一定程度上通过肠道微生物相关的5 -羟色胺和褪黑素的合成，保护了高脂肪喂养引起的卵泡闭锁。该结果为预防高能饮食对人类生育能力的负面影响提供了思路。
外文摘要：To investigate the effects of dietary fibre on follicular atresia in pigs fed a high-fat diet, we fed thirty-two prepubescent gilts a basal diet (CON) or a CON diet supplemented with 300 g/d dietary fibre (fibre), 240 g/d soya oil (SO) or both (fibre + SO). At the 19th day of the 4th oestrus cycle, gilts fed the SO diet showed 112 % more atretic follicles and greater expression of the apoptotic markers, Bax and caspase-3, and these effects were reversed by the fibre diet. The abundance of SCFA-producing microbes was decreased by the SO diet, but this effect was reversed by fibre treatment. Concentrations of serotonin and melatonin in the serum and follicular fluid were increased by the fibre diet. Overall, dietary fibre protected against high fat feeding-induced follicular atresia at least partly via gut microbiota-related serotonin-melatonin synthesis. These results provide insight into preventing negative effects on fertility in humans consuming a high-energy diet.
外文关键词：Dietary fibre; Microbiota; Serotonin; Melatonin; Follicular atresia
作者：Zhuo, Y；Cao, M；Gong, YC；Tang, LC；Jiang, XM；Li, Y；Yang, M；Xu, SY；Li, J；Che, LQ；Lin, Y；Feng, B；Fang, ZF；Wu, D
作者单位：Sichuan Agr Univ
期刊名称：BRITISH JOURNAL OF NUTRITION
期刊影响因子：3.334
出版年份：2021
出版刊次：125
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