中文摘要：本研究鉴定了两种噬菌体phi FG02和phi CO01对临床分离的鲍曼不动杆菌的拮抗作用，并确定细菌荚膜是两种噬菌体的受体。噬菌体抗性突变体负责荚膜生物合成的基因存在功能缺失突变，导致荚膜丢失和噬菌体吸附中断。抗噬菌体菌株对人类补体、β-内酰胺类抗生素和替代性噬菌体重新敏感，在体内表现出适应性下降。本文利用鲍曼不动杆菌感染的小鼠模型，证明了噬菌体疗法的有效性。
外文摘要：We characterized two bacteriophages, phi FG02 and phi CO01, against clinical isolates of Acinetobacter baumannii and established that the bacterial capsule is the receptor for these phages. Phage-resistant mutants harboured loss-of-function mutations in genes responsible for capsule biosynthesis, resulting in capsule loss and disruption of phage adsorption. The phage-resistant strains were resensitized to human complement, beta-lactam antibiotics and alternative phages and exhibited diminished fitness in vivo. Using a mouse model of A. baumannii infection, we showed that phage therapy was effective.
外文关键词：Phage therapy；Acinetobacter baumannii；bacterial capsule；phage receptors；antimicrobial resistance
作者：Altamirano, FG；Forsyth, JH；Patwa, R；Kostoulias, X；Trim, M；Subedi, D；Archer, SK；Morris, FC；Oliveira, C；Kielty, L；Korneev, D；O'Bryan, MK；Lithgow, TJ；Peleg, AY；Barr, JJ
作者单位：Monash Univ
期刊名称：NATURE MICROBIOLOGY
期刊影响因子：15.54
出版年份：2020
出版刊次：
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