中文摘要：尽管提出了不少用于控制气管内导管上生物膜形成的方案，但用应用噬菌体去除和防止器械表面铜绿假单胞菌生物膜的科学数据不多。本研究通过高含量筛选试验评估了五种噬菌体的抗生物膜能力。研究发现，经噬菌体处理后生物膜明显减少，尤其是多重耐药菌株。选择两种噬菌体作为鸡尾酒成分，并在动态生物膜模型中测试鸡尾酒对预防气管导管表面定植的能力。用不同的铜绿假单胞菌测试噬菌体涂层管。通过菌落形成单位计数、代谢活性评估和生物膜形态观察，监测24-168h生物膜的生长情况。结果表明，噬菌体鸡尾酒促进了细菌定植的差异;但这种差异与菌株有关；噬菌体鸡尾酒涂层并没有实质性地促进代谢活性变化；扫描电镜观察发现，对照组的生物膜细胞浓度较高，而在噬菌体鸡尾酒涂层管上可见塔状结构。这些结果表明，随着新包衣策略的发展，噬菌体处理在控制气管导管相关生物膜方面具有潜力。
外文摘要：Although different strategies to control biofilm formation on endotracheal tubes have been proposed, there are scarce scientific data on applying phages for both removing and preventing Pseudomonas aeruginosa biofilms on the device surface. Here, the anti-biofilm capacity of five bacteriophages was evaluated by a high content screening assay. We observed that biofilms were significantly reduced after phage treatment, especially in multidrug-resistant strains. Considering the anti-biofilm screens, two phages were selected as cocktail components, and the cocktail's ability to prevent colonization of the endotracheal tube surface was tested in a dynamic biofilm model. Phage-coated tubes were challenged with different P. aeruginosa strains. The biofilm growth was monitored from 24 to 168 h by colony forming unit counting, metabolic activity assessment, and biofilm morphology observation. The phage cocktail promoted differences of bacterial colonization; nonetheless, the action was strain dependent. Phage cocktail coating did not promote substantial changes in metabolic activity. Scanning electron microscopy revealed a higher concentration of biofilm cells in control, while tower-like structures could be observed on phage cocktail-coated tubes. These results demonstrate that with the development of new coating strategies, phage therapy has potential in controlling the endotracheal tube-associated biofilm.
外文关键词：bacteriophage; biofilm; Pseudomonas aeruginosa; endotracheal tube
作者：Oliveira, VC；Macedo, AP；Melo, LDR；Santos, SB；Hermann, PRS；Silva-Lovato, CH；Paranhos, HFO；Andrade, D；Watanabe, E
作者单位：Univ Sao Paulo；Univ Minho；Univ Brasilia
期刊名称：ANTIBIOTICS-BASEL
期刊影响因子：3.893
出版年份：2021
出版刊次：1
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