中文摘要：特应性皮炎常伴有金黄色葡萄球菌的异常过度生长，金黄色葡萄球菌，是一种条件致病菌，也是皮肤感染的常见原因。尽管使用抗生素对金黄色葡萄球菌有效，但由此导致健康微生物群的减少和耐药细菌的出现仍令人担忧。噬菌体疗法可能是一种治疗特应性皮炎而不扰乱菌群结构的有效策略。本文用小鼠模型研究了金黄色葡萄球菌噬菌体SaGU1是否可以抵抗由金黄色葡萄球菌引起的过敏性加重。结果表明，在小鼠背部皮肤注射SaGU1，可以减少金黄色葡萄球菌数量，缓解由金黄色葡萄球菌引起的症状加重。此外，应用SaGU1可改善金黄色葡萄球菌介导的特应性皮炎在IgE血浆浓度和组织病理学表现的恶化。研究还发现，在液体培养感染实验中，健康皮肤中典型的表皮共生菌表皮葡萄球菌，与金黄色葡萄球菌共培养后，显著降低了耐SAGU1的金黄色葡萄球菌的出现。可见，临床上使用SaGU1噬菌体治疗特应性皮炎具有很好的前景。
外文摘要：Atopic dermatitis is accompanied by the abnormal overgrowth of Staphylococcus aureus, a common cause of skin infections and an opportunistic pathogen. Although administration of antibiotics is effective against S. aureus, the resulting reduction in healthy microbiota and the emergence of drug-resistant bacteria are of concern. We propose that phage therapy can be an effective strategy to treat atopic dermatitis without perturbing the microbiota structure. In this study, we examined whether the S. aureus phage SaGU1 could be a tool to counteract the atopic exacerbation induced by S. aureus using an atopic mouse model. Administration of SaGU1 to the back skin of mice reduced both S. aureus counts and the disease exacerbation caused by S. aureus. Furthermore, the S. aureus-mediated exacerbation of atopic dermatitis with respect to IgE plasma concentration and histopathological findings was ameliorated by the application of SaGU1. We also found that Staphylococcus epidermidis, a typical epidermal symbiont in healthy skin, significantly attenuated the emergence of SaGU1-resistant S. aureus under co-culture with S. aureus and S. epidermidis in liquid culture infection experiments. Our results suggest that phage therapy using SaGU1 could be a promising clinical treatment for atopic dermatitis.
外文关键词：bacteriophage；phage therapy；atopic dermatitis；Staphylococcus aureus； Staphylococcus epidermidis
作者：Shimamori, Y；Mitsunaka, S；Yamashita, H；Suzuki, T；Kitao, T；Kubori, T；Nagai, H；Takeda, S；Ando, H
作者单位：Gifu Univ；Gunma Univ；Univ Ryukyus
期刊名称：VIRUSES-BASEL
期刊影响因子：3.816
出版年份：2021
出版刊次：1
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