中文摘要：粪肠球菌是生物膜相关多菌感染的主要医院内病原菌。本研究旨在通过基因组分析了解该病原菌的致病性和生物膜决定因素。对一株形成强生物膜、临床分离、缺失Fsr群体信号系统的粪肠球菌SK460进行基因组测序，并对一组致病性生物膜形成菌和非致病性弱生物膜形成菌进行比较基因组学研究。研究发现一个有关与致病性相关的毒力和粘附相关因子库。CRISPR-Cas系统的缺失促进了信息素反应应答质粒、致病岛和噬菌体的获得。综合分析发现了一组专门编码多糖裂解酶、糖磷酸转移酶系统、噬菌体蛋白和转录调控因子的辅助基因。本研究确定了一组致病性生物膜形成的特定特异性基因，以它们为目标靶标，从而有助于将来可以反过来帮助开发治疗肠球菌感染的方法。
外文摘要：Aim: Enterococcus faecalis is a leading nosocomial pathogen in biofilm-associated polymicrobial infections. The study aims to understand pathogenicity and biofilm determinants of the pathogen by genome analysis. Methodology: Genome sequencing of a strong biofilm forming clinical isolate E. faecalis SK460 devoid of Fsr quorum-signaling system, was performed and comparative genomics was carried out among a set of pathogenic biofilm formers and nonpathogenic weak biofilm formers. Results: Analysis revealed a pool of virulence and adhesion related factors associated with pathogenicity. Absence of CRISPR-Cas system facilitated acquisition of pheromone responsive plasmid, pathogenicity island and phages. Comprehensive analysis identified a subset of accessory genes encoding polysaccharide lyase, sugar phosphotransferase system, phage proteins and transcriptional regulators exclusively in pathogenic biofilm formers. Conclusion: The study identified a set of genes specific to pathogenic biofilm formers and these can act as targets which in turn help to develop future treatment endeavors against enterococcal infections.
外文关键词：accessory genes；biofilm；Enterococcus faecalis；pan-genome；pathogenicity
作者：Suryaletha, K；Chandrika, SK；Thomas, S
作者单位：Rajiv Gandhi Ctr Biotechnol
期刊名称：FUTURE MICROBIOLOGY
期刊影响因子：2.907
出版年份：2021
出版刊次：3
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