中文摘要：以噬菌体UFV-AREG1为模式生物，通过挤出方式评估采用不同亲水胶体的评估其挤压包封效果。噬菌体载菌微球用纯海藻酸盐、海藻酸盐与卡拉胶、壳聚糖或乳清蛋白的混合物制备。包封液的流动特性液呈现为非牛顿假塑性流体的流动特性，亲水胶体浓度不影响微球形貌，海藻酸盐-壳聚糖溶液的流动稠度指数较高，流动特性指数较低。包封率约为99%，利用异硫氰酸荧光素标记的包封噬菌体经共聚焦显微摄影，显示病毒颗粒在微球内均匀分布。即使置于pH 2.5的条件下2小时，噬菌体仍能在海藻酸盐-乳清蛋白微球中存活。直接在模拟肠液(pH 6.8)中培养微球，5分钟后UFV-AREG1噬菌体的释放量仅为50%，即使用模拟胃液预处理也是如此。包封后噬菌体在冷藏条件下仍能存活5个月。包封的UFV-AREG1噬菌体对脱水很敏感，表明需要使用保护剂。本研究首次将噬菌体包封在海藻酸-卡拉胶微球中，以及以海藻酸-壳聚糖为微球亲水胶体；还提出了一种用海藻酸盐-乳清蛋白包封噬菌体的新方法。该体系对UFV-AREG1噬菌体的包封效果良好，可用于多种生物活性化合物的包封。
外文摘要：The bacteriophage UFV-AREG1 was used as a model organism to evaluate the encapsulation via extrusion using different hydrocolloids. Pure alginate [0.75%, 1.0%, 1.5% and 2.0% (m/v)] and mixtures of alginate [0.75% or 1.0% (m/v)] with carrageenan [1.25% (m/v)], chitosan [0.5% (m/v)], or whey protein [1.5% (m/v)] were used to produce bacteriophage-loaded beads. The encapsulating solutions presented flow behavior of non-Newtonian pseudoplastic fluids and the concentration of hydrocolloid did not influence (p > 0.05) the morphology of the beads, except for alginate-chitosan solutions, which presented the higher flow consistency index (K) and the lower flow behavior index (n). The encapsulation efficiency was about 99% and the confocal photomicrography of the encapsulated bacteriophages labeled with fluorescein isothiocyanate showed homogenous distribution of the viral particles within the beads. The phages remained viable in the beads of alginate-whey protein even when submitted to pH 2.5 for 2 h. Beads incubated directly in simulated intestinal fluid (pH 6.8) resulted in a minimal of 50% release of the UFV-AREG1 phages after 5 min, even when previously submitted to the simulated gastric fluid (pH 2.5). Encapsulation enabled phages to remain viable under refrigeration for five months. Encapsulated UFV-AREG1 phages were sensitive to dehydration, suggesting the need for protective agents. In this study, for the first-time bacteriophages were encapsulated in alginate-carrageenan beads, as well as alginate-chitosan as a bead-forming hydrocolloid. In addition, a novel procedure for encapsulating bacteriophages in alginate-whey protein was proposed. The assembled system showed efficiency in the encapsulation of UFV-AREG1 bacteriophages using different hydrocolloids and has potential to be used for the entrapment of a variety of bioactive compounds.
外文关键词：Escherichia coli O157:H7；Phage therapy；Rheology；Carrageenan；Chitosan；Whey protein
作者：Batalha, LS；Gontijo, MTP；Teixeira, AVND；Boggione, DMG；Lopez, MES；Eller, MR；Mendonca, RCS
作者单位：Univ Fed Vicosa UFV；Univ Estadual Campinas；Univ Cordoba UNICORDOBA
期刊名称：FOOD RESEARCH INTERNATIONAL
期刊影响因子：4.972
出版年份：2021
出版刊次：139
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