中文摘要：本研究分析了Listex P100噬菌体对稳定期和指数期对不同浓度单核细胞增生李斯特菌的抑菌和杀菌能力。室温下用酪蛋白酸钠、海藻酸钠明胶混合物、聚乙烯醇（PVOH）三种基质制备Listex P100生物膜，并对这些活性生物降解膜进行了光学、结构、热研究，还研究了其抗对单核细胞增生李斯特菌的抗菌性能。结果表明，噬菌体的加入对聚合物的形态、颜色、透明度和热稳定性没有影响；抗菌活性分析表明，噬菌体对稳定期单核细胞增生李斯特菌具有较高的抗菌能力；30℃孵育24小时后，生物膜抗菌能力接近1log。PVOH生物膜在制冷条件下更有效，孵育8天后，其抗菌能力达到2log。使用这些膜作为食品涂层或包装成分，可以提高食品的安全性，防止致病微生物，如单核细胞增生李斯特菌的生长。
外文摘要：The inhibitory and bactericidal capacity of Listex P100 bacteriophage has been studied against different concentrations of Listeria monocytogenes in stationary and exponential phases. Three different matrices were employed to developed films incorporating Listex P100: (1) sodium caseinate, (2) sodium alginate mixed with gelatin, and (3) polyvinyl alcohol (PVOH). All the films were successfully developed by casting at room temperature. These active biodegradable films were optical, structural, and thermally characterized, and their antimicrobial capacities against L. monocytogenes were studied. The incorporation of phages did not affect the morphology, colour, opacity, and thermal stability of polymers. The antimicrobial analysis revealed the bacteriophage presented a high antimicrobial capacity against L. monocytogenes in the stationary phase (4.40 and 6.19 log reduction values or bactericide effect depending on the initial inoculum of the pathogen). Developed films showed antimicrobial capacity close to 1 log after 24 h of incubation at 30 degrees C. The effectiveness of PVOH films was greater under refrigeration conditions, reaching 2 log reduction after eight days of incubation. The use of these films as a coating in a food or as part of a packaging could improve food safety against the growth of pathogenic microorganisms such as Listeria monocytogenes.
 
外文关键词：bateriophage；Listeria monocytogenes；antimicrobial films；polyvinyl alcohol；sodium caseinate；sodium alginate
作者：de Dicastillo, CL；Settier-Ramirez, L；Gavara, R；Hernandez-Munoz, P；Carballo, GL
作者单位：Univ Santiago de Chile USACH；IATA CSIC
期刊名称：POLYMERS
期刊影响因子：3.426
出版年份：2021
出版刊次：3
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