中文摘要：曾有人有学者用M13噬菌体颜色传感器鉴别农产品（大蒜、洋葱和紫苏）的地理来源，识别准确度在88.6%-94.0%之间，属于可接受范围。为了进一步提高准确度，本研究提出了三种含有不同氨基酸残基的M13噬菌体颜色传感器（Wild野生型传感器：谷氨酸（E）-甘氨酸（G）-天冬氨酸（D），WHW传感器：色氨酸（W）-组氨酸（H）-色氨酸（W），4E传感器：四个重复的谷氨酸（E））的应用。本研究的前提是：通过结合样品气味与所使用的颜色传感器的不同的相互作用所产生的每个传感器采集的互为特征和互补RGB信号，提高样品识别的可能性。当单独使用单个颜色传感器时，基于支持向量机（SVM）的大蒜、洋葱和紫苏样品的识别准确率分别为91.8-94.0%、88.6-90.3%和89.8-92.1%；整合三种颜色传感器采集的所有RGB信号后，精度分别提高到98.0%、97.5%和97.1%。可见，该策略对提高样本的可辨识性是有效的。为了进一步研究每个颜色传感器对气味分子的不同反应，还使用每个颜色传感器测量了样品中的典型气味成分（烯丙基二硫、烯丙基甲基二硫和紫苏醛），并对RGB信号的差异进行了分析。
外文摘要：A single M13 bacteriophage color sensor was previously utilized for discriminating the geographical origins of agricultural products (garlic, onion, and perilla). The resulting discrimination accuracy was acceptable, ranging from 88.6% to 94.0%. To improve the accuracy further, the use of three separate M13 bacteriophage color sensors containing different amino acid residues providing unique individual color changes (Wild sensor: glutamic acid (E)-glycine (G)-aspartic acid (D), WHW sensor: tryptophan (W)-histidine (H)-tryptophan (W), 4E sensor: four repeating glutamic acids (E)) was proposed. This study was driven by the possibility of enhancing sample discrimination by combining mutually characteristic and complimentary RGB signals obtained from each color sensor, which resulted from dissimilar interactions of sample odors with the employed color sensors. When each color sensor was used individually, the discrimination accuracy based on support vector machine (SVM) ranged from 91.8-94.0%, 88.6-90.3%, and 89.8-92.1% for garlic, onion, and perilla samples, respectively. Accuracy improved to 98.0%, 97.5%, and 97.1%, respectively, by integrating all of the RGB signals acquired from the three color sensors. Therefore, the proposed strategy was effective for improving sample discriminability. To further examine the dissimilar responses of each color sensor to odor molecules, typical odor components in the samples (allyl disulfide, allyl methyl disulfide, and perillaldehyde) were measured using each color sensor, and differences in RGB signals were analyzed.
外文关键词：bacteriophage-based structural color sensor；multiple color sensors； discrimination of geographical origin；garlic；onion；perilla
作者：Seol, D；Jang, D；Cha, K；Oh, JW；Chung, H
作者单位：Hanyang Univ；Pusan Natl Univ
期刊名称：SENSORS
期刊影响因子：3.275
出版年份：2021
出版刊次：3
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