中文摘要：有些细菌种类是致命致病菌，在全球具有很高的发病率和死亡率。细菌病原体传播的主要媒介包括食物、水、乳制品、家畜和人类的相互作用。这种细菌的早期检测对于减少细菌性疾病的风险和确保早期诊断至关重要。大多数常规微生物、生物化学检测方法都费时费力、需要熟练的操作人员，而结果还不一定准确。利用酶、抗体、核酸等敏感而特异的生物识别元素，已开发出各种分子诊断工具及分析方法，并广泛用于致病菌检测。检测病原体理想的生物识别元素应具有高特异性、稳定性、敏感性、选择性、快速、简便、经济有效等特性。噬菌体能满足这些条件，可用作生物识别元素以替代现有的分子探针，开发出经济、特异、快速、灵敏、可靠的病原菌检测平台(传感器和分析方法)。本综述详细介绍了噬菌体生物学特性及有尾噬菌体表面的各种识别位点和受体结合蛋白，这些位点可特异性识别细菌。它突出了细菌表面结合和附着特定噬菌体的结构和受体。细菌和噬菌体的这些特征为建立基于噬菌体的细菌检测方法提供了基础，这些方法包括噬菌体诱导的细菌溶解、固定在传感器表面的噬菌体、荧光标记噬菌体、噬菌体共轭量子点和重组噬菌体，特别是通过光学和电化学传感器系统监测。
外文摘要：Some bacterial species are deadly disease-causing pathogens with high morbidity and mortality in humans worldwide. Key interfaces in the transmission of bacterial pathogens include food, water, dairy products, peri-domestic animals, and human interplay. Early-stage detection of such bacteria is crucial in minimizing the risk of bacterial diseases and ensuring early diagnosis. Majority of the conventional microbiological and biochemical detection methods are laborious, require skilled individuals, and are not always accurate. Various molecular diagnostic tools and assays, utilizing sensitive and specific biorecognition elements, such as enzymes, antibodies, and nucleic acids, have been developed and widely used for the detection of pathogenic bacteria. An ideal biorecognition element for the detection of pathogens should be highly specific, stable, sensitive, selective, rapid, easily available, and cost-effective. Bacteriophages, which meet such prerequisites, may be used as biorecognition element alternatives to the currently available molecular probes in the development of cost-effective, specific, quick, sensitive, and reliable platforms (sensors and assays) for the detection of bacterial pathogens. This review details bacteriophage biology and various recognition sites and receptor-binding proteins on the surfaces of tailed phages, which can be used as the recognition sites for specific bacterial detection. It highlights structures and receptors on the surface of bacteria for binding and attachment of specific phages. These features of bacteria and phages provide a basis for establishing methodologies for phage-based bacterial detection, including phage-induced bacterial lysis, phages immobilized on a transducer surface, fluorescently labelled phages, phage-conjugated quantum dots, and recombinant reporter phages, particularly monitored through optical and electrochemical transducer systems.
外文关键词：Bacterial detection；Phage recognitions sites；Bacterial receptors；Phage-based biosensors；Fluorescently labelled phages；Recombinant phages；Reporter phages
作者：Hussain, W；Ullah, MW；Farooq, U；Aziz, A；Wang, SQ
作者单位：Huazhong Univ Sci & Technol
期刊名称：BIOSENSORS & BIOELECTRONICS
期刊影响因子：10.257
出版年份：2021
出版刊次：177
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