中文摘要：本研究采用海藻酸钠和酪蛋白酸钠挤压法对可侵染肠沙门氏菌的噬菌体Felix O1进行微囊化。通过对噬菌体微囊化工艺参数的优化，研究了微囊化效率最高、耐低pH值和周长最小的微囊化工艺；采用响应面法优化包封工艺；通过改变微胶囊混合料中海藻酸钠和酪蛋白酸钠含量的比例和挤压过程中的振动频率，获得了最佳挤压工艺条件。模型预测的最高效微囊化效率(OptME)值为：振动频率值1.61 kHz，微胶囊混合料中海藻酸钠含量1.86%，酪蛋白酸钠含量3.26%。在频率值4.50 kHz，微胶囊混合料含2.00%海藻酸钠和2.69%酪蛋白酸钠时，微囊化噬菌体Felix O1滴度(OptR)的还原率最低。测定了优化条件下制备的噬菌体felix O1微囊在模拟胃液和肠液中的活性。在模拟胃液中，OptR微胶囊中检测到felix O1噬菌体滴度下降最低。在最佳条件下制备的微胶囊均在模拟肠液中成功释放。表明基于响应面法获得的最佳条件可用于在海藻酸钠和酪蛋白酸钠溶液中用挤出法进行噬菌体felix O1微囊化。
外文摘要：In this study, phage Felix O1 which has ability to infect Salmonella enterica, was microencapsulated by using extrusion method with sodium alginate and sodium caseinate. The formation of microcapsules with the highest microencapsulation efficiency, the highest resistance to low pH and the smallest perimeter was investigated by optimization of process variables applied in bacteriophage microencapsulation. Response Surface Methodology was used for the optimization of the encapsulation process. The optimum conditions were obtained by changing the ratio of sodium alginate content to sodium caseinate content of microencapsulating mixture and the vibration frequency during extrusion process. The optimum conditions for the highest microencapsulation efficiency (OptME) value predicted by the model were: vibration frequency value of 1.61 kHz, the sodium alginate content of 1.86% and sodium caseinate content of 3.26% in the microencapsulating mixture. The lowest reduction rate of microencapsulated phage Felix O1 titers (OptR) was achieved at the frequency value of 4.50 kHz, and with using the microencapsulating mixture contained 2.00% of sodium alginate and 2.69% of sodium caseinate. The activity of microencapsulated phage Felix O1 produced under optimized conditions in the simulated gastric and intestinal fluids was determined. The lowest decrease in the phage titers of Felix O1 was detected in the microcapsules produced for OptR, in the simulated gastric fluid. All microcapsules produced under optimum conditions were successfully released in simulated intestinal fluid. In conclusion, the optimum conditions obtained by the Response Surface Methodology can be used to phage Felix O1 microencapsulation in solutions of sodium alginate and sodium caseinate with extrusion method.
外文关键词：Felix O1；Salmonella enterica；Microencapsulation；Simulated gastric fluids；Simulated intestinal fluid
作者：Ergin, F；Atamer, Z；Gocer, EMC；Demir, M；Hinrichs, J；Kucukcetin, A
作者单位：Akdeniz Univ；Univ Hohenheim
期刊名称：FOOD HYDROCOLLOIDS
期刊影响因子：7.053
出版年份：2021
出版刊次：113
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