中文摘要：生物界面相互作用的有效控制在从细胞培养工具到生物传感器和植入式医疗设备等生物医学具有广泛应用。在这些应用中，需要细胞和材料表面之间高度特异的相互作用。精细控制这些相互作用一方面需要减少或阻止非特异性的相互作用，另一方面需要显示能被细胞外受体识别的高度特异性信号。我们最近开发了一种用2-羟丙基丙烯酰胺与N -二苯甲酮丙烯酰胺的光反应共聚物单步应用的超低污染涂料。本文通过将可聚合的肽单体合并到这些共聚物中来扩展这种方法。通过噬菌体展示鉴定了QQGWF序列与CD44的结合基序，首次合成了单体QQGWFGAGK(丙烯酰胺)和丙烯酰胺- GAGQQGWF。研究表明，由于CD44表达的差异，使用QQGWFGAGK(丙烯酰胺)单体制备的UV交联涂层能够有效地在HepG2和HEK293细胞存在的情况下选择性结合hMSC；BCA蛋白结合试验和大肠杆菌附着试验表明，超过24小时肽修饰涂层仍能保持其低生物污染的特性。该方法利用跨膜蛋白CD44在多种疾病中高表达，提供了一种替代传统整合素介导的细胞表面选择性结合方法，打开了诊断应用的新大门。
外文摘要：The effective control of biointerfacial interactions is of outstanding interest in a broad range of biomedical applications, ranging from cell culture tools to biosensors and implantable medical devices. For many of these applications, highly specific interactions between cells and material surfaces are desired. Sophisticated control over these interactions requires reducing or preventing non-specific interactions on the one hand and displaying highly specific signals that can be recognized by extracellular receptors on the other. We have recently developed ultra-low fouling coatings that can be applied in a single step using photoreactive copolymers of 2-hydroxypropyl acrylamide and N-benzophenone acrylamide. Here, we have expanded this approach by incorporating polymerizable peptide monomers into these copolymers. The monomers QQGWFGAGK(acrylamide) and acrylamide-GAGQQGWF were synthesized after identifying the QQGWF sequence as a binding motif for CD44 by phage display for the first time. Our results demonstrate that UV-crosslinked coatings fabricated using the QQGWFGAGK(acrylamide) monomer are effective at selectively binding hMSC in the presence of HepG2 and HEK293 cells due to the difference in CD44 expression. Our results also demonstrate that the peptide modified coatings retain their low biofouling character using a BCA protein binding assay as well as an E. coli bacterial attachment assay over a 24 h period. Our approach provides an alternative to traditional integrin-mediated selective cell binding on surfaces and opens the door to new diagnostic applications, exploiting the fact that the transmembrane protein CD44 is highly expressed in multiple diseases.
外文关键词：Coatings；Biofouling；Phage display；Peptide；CD44；Biointerface
作者：Yoshikawa, C；Nakaji-Hirabayashi, T；Nishijima, N；Nonsuwan, P；Toh, RJ；Kowalczyk, W；Thissen, H
作者单位：Natl Inst Mat Sci NIMS；Univ Toyama；CSIRO Mfg
期刊名称：MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
期刊影响因子：5.88
出版年份：2021
出版刊次：120
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