中文摘要：铜绿假单胞菌是一种条件致病菌，具有多种表型，进化潜力高，能适应各种自然生境。由于生物通常生活在从野外到宿主体内的空间异质性和多微生物环境中，对非生物(空间异质性)和生物因素(种间相互作用)的适应性是其增殖的关键过程。然而，目前对铜绿假单胞菌与其他物种相关的空间异质性环境的适应过程的认识还很有限。研究表明，决定铜绿假单胞菌PAO1在空间异质性环境中，与已知的体内共存的金黄色葡萄球菌的进化动力学有两个不同的核心进化轨迹：（i）生物膜形成的增加和（ii）PAO1基因组中保留的丝状噬菌体的抗感染性。在实验室进化实验中，经常选择超级生物膜和/或菌毛缺陷型耐噬菌体变异株，表明它们是空间结构条件下的关键适应性性状。而金黄色葡萄球菌的存在对这些变异株的出现和维持只有微小的影响。本研究说明了铜绿假单胞菌的关键适应性特征，表明空间异质性带来的强大选择压力可能会超过种间相互作用的影响。
外文摘要：An opportunistic pathogen Pseudomonas aeruginosa has a versatile phenotype and high evolutionary potential to adapt to various natural habitats. As the organism normally lives in spatially heterogeneous and polymicrobial environments from open fields to the inside of hosts, adaptation to abiotic (spatial heterogeneity) and biotic factors (interspecies interactions) is a key process to proliferate. However, our knowledge about the adaptation process of P. aeruginosa in spatially heterogeneous environments associated with other species is limited. We show herein that the evolutionary dynamics of P. aeruginosa PAO1 in spatially heterogeneous environments with Staphylococcus aureus known to coexist in vivo is dictated by two distinct core evolutionary trajectories: (i) the increase of biofilm formation and (ii) the resistance to infection by a filamentous phage which is retained in the PAO1 genome. Hyperbiofilm and/or pili-deficient phage-resistant variants were frequently selected in the laboratory evolution experiment, indicating that these are key adaptive traits under spatially structured conditions. On the other hand, the presence of S. aureus had only a marginal effect on the emergence and maintenance of these variants. These results show key adaptive traits of P. aeruginosa and indicate the strong selection pressure conferred by spatial heterogeneity, which might overwhelm the effect of interspecies interactions.
外文关键词：Pseudomonas aeruginosa；evolution；spatial heterogeneity；interspecies interaction；biofilm；Staphylococcus aureus
作者：Yamamoto, K；Kusada, H；Kamagata, Y；Tamaki, H
作者单位：Natl Inst Adv Ind Sci & Technol；AIST；Univ Tsukuba；Univ Tokyo
期刊名称：MICROORGANISMS
期刊影响因子：4.152
出版年份：2021
出版刊次：3
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