中文摘要：为应对耐药菌的出现，本研究采用本地分离的针对H5α大肠杆菌的噬菌体(HZJ)作为抗菌剂，制作伤口敷料样品。用物理方法将这些噬菌体嵌入到海藻酸水凝胶样品中，使它们可以在感染过程中释放，并保持它们具有裂解活性的尾部的自由。HZJ噬菌体有20分钟的潜伏期，在pH6-pH9、45℃以下的温度保持稳定。向大肠杆菌培养物中添加该噬菌体，2小时内抑制了超过99%的细菌生长。使用3D打印技术，用嵌入了噬菌体的水凝胶纤维制备多孔的伤口敷料。研究发现，包埋后保留了大部分噬菌体的裂解活性(85%-90%)。嵌入样品后，HZJ在2h内裂解了57%-67%的细菌，抗菌效果至少持续24h。2h内释放的少量噬菌体能够快速复制并有效裂解大部分细菌宿主。包埋噬菌体的海藻酸盐样品在24小时内释放出10%的噬菌体颗粒。扫描电镜技术显示，与无噬菌体的样品相比，与细菌接触2h后，包埋有噬菌体的样品中观察到的大肠杆菌细胞较少。包埋了噬菌体的样品对L929细胞没有细胞毒性。海藻酸盐水凝胶中HZJ的存在促进了细胞的生长和对样品的附着力。此外，噬菌体的存在并没有改变试样的拉伸强度和系数。本研究制备了一种抗菌敷料，能够延缓释放裂解噬菌体，有效地抑制细菌生长长达24小时。本方法可减少抗生素和其他添加剂在传统敷料中的使用。
外文摘要：To combat the emergence of drug-resistant bacteria, a locally isolated bacteriophage (HZJ) targeting H5 alpha Escherichia coli was used as an antibacterial agent to make wound dressing samples in this study. The phages were physically embedded within an alginate hydrogel sample so that they could later be released with their tails being free during the infection process, which preserves their lytic activity. The HZJ phage isolated in the study have a 20 min latent period and are stable between pH 6 and pH 9 and at temperatures below 45 degrees C. The addition of phage to an E. coli culture suppressed over 99% of bacterial growth in 2-h (p < 0.001). Phage-embedded hydrogel fibers were used to create porous wound dressing material using three-dimensional (3D) printing. The majority of phage lytic activity (85%-90%) was preserved after encapsulation. After they were embedded in samples, HZJ lysed 57% to 67% of bacteria (p < 0.001) within 2 h and the antibacterial effects lasted at least 24 h. The small amount of phage released in 2 h was able to quickly replicate and effectively lysed the majority of the bacterial hosts. Phage-embedded alginate samples released 10% of its incorporated phage particles in 24 h. The SEM micrographs show that, compared to phage-free samples, fewer E.coli cells were observed on phage-embedded samples 2 h after bacteria were exposed to the samples. The phage-embedded sample was not cytotoxic to L929 cells. The presence of HZJ in alginate hydrogel promoted cell growth (p < 0.01) and adhesion to the samples. Further, the existence of phage did not alter the tensile strength and modulus of samples (p > 0.05). An antibacterial dressing capable of slowly releasing lytic phages and effectively suppressing bacterial growth for up to 24 h was produced in this study. This model represents an attractive means to reduce use of antibiotics and other additives in conventional dressings.
外文关键词：Antibacterial wound dressing；Controlled release；Phage nanoparticles；Alginate；Three-dimensional (3D) plotting
作者：Shen, HY；Liu, ZH；Hong, JS；Wu, MS；Shiue, SJ；Lin, HY
作者单位：Natl Taipei Univ Technol；Taipei Med Univ
期刊名称：JOURNAL OF CONTROLLED RELEASE
期刊影响因子：7.727
出版年份：2021
出版刊次：331
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