中文摘要：噬菌体展示技术是一项重要而有效的分子生物学技术，自1985年创建以来，一直是研究界不可缺少的技术。由于大量的核苷酸片段可能被克隆到噬菌体基因组中，噬菌体文库中可能含有数百万甚至几十亿个独特的肽配体。噬菌体展示的基础，配体-受体相互作用，已广泛应用于噬菌体表面抗原表位定位和抗原递呈，并通过基于亲和选择的生物筛选策略，筛选新的候选疫苗。这种多功能技术在过去三十年中得到了极大的改进，产生了不同的组合肽展示平台。由此开发的新诊断工具已用于由致病菌引起的情况，包括细菌和致命病毒，如寨卡病毒、埃博拉病毒、亨德拉病毒、尼帕病毒、汉塔病毒、中东呼吸综合征病毒和非典病毒。在当前冠状病毒病（COVID-19）大流行的形势下，寻找中和抗体正促使研究人员开发治疗SARS-CoV-2的候选药物。噬菌体展示技术是一种重要的抗体筛选技术，本文简要综述了噬菌体展示技术的最新应用，重点介绍了噬菌体展示技术在冠状病毒疾病诊断和治疗方面的研究进展。该项技术有望补充对宿主-病原体相互作用的研究，并协助研发冠状病毒新药物。
外文摘要：Phage display is one of the important and effective molecular biology techniques and has remained indispensable for research community since its discovery in the year 1985. As a large number of nucleotide fragments may be cloned into the phage genome, a phage library may harbour millions or sometimes billions of unique and distinctive displayed peptide ligands. The ligand-receptor interactions forming the basis of phage display have been well utilized in epitope mapping and antigen presentation on the surface of bacteriophages for screening novel vaccine candidates by using affinity selection-based strategy called biopanning. This versatile technique has been modified tremendously over last three decades, leading to generation of different platforms for combinatorial peptide display. The translation of new diagnostic tools thus developed has been used in situations arising due to pathogenic microbes, including bacteria and deadly viruses, such as Zika, Ebola, Hendra, Nipah, Hanta, MERS and SARS. In the current situation of pandemic of Coronavirus disease (COVID-19), a search for neutralizing antibodies is motivating the researchers to find therapeutic candidates against novel SARS-CoV-2. As phage display is an important technique for antibody selection, this review presents a concise summary of the very recent applications of phage display technique with a special reference to progress in diagnostics and therapeutics for coronavirus diseases. Hopefully, this technique can complement studies on host-pathogen interactions and assist novel strategies of drug discovery for coronaviruses.
作者：Anand, T；Virmani, N；Bera, BC；Vaid, RK；Vashisth, M；Bardajatya, P；Kumar, A；Tripathi, BN
作者单位：ICAR NRCE；CCS Haryana Agr Univ；Guru Jambheshwar Univ；Indian Council Agr Res
期刊名称：CURRENT MICROBIOLOGY
期刊影响因子：1.746
出版年份：2021
出版刊次：4
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