中文摘要：由于裂解噬菌体与细菌物种之间存在着复杂的相互作用，它们在微生物生态学，特别是在水生生境中发挥着关键作用。噬菌体治疗将噬菌体作为抗菌剂，在水环境中的使用虽然一直在增加，但最新研究表明，由于革兰氏阴性菌噬菌体耐药菌的发展，该疗法存在缺陷。利用生化、代谢和分子适应策略，已经证明海洋弧菌的获得性噬菌体抗性是一个非常复杂的过程。这一多组学方法的结果结合溶藻弧菌耐噬菌体菌株的转录组和代谢组分析，证实了这一前景。本研究为噬菌体耐受菌株减少噬菌体受体ompF、lamB和btuB的表达提供了新思路。编码天然营养通道的基因，如rbsA、ptsG、tryP、livH、lysE和hisp也存在这种情况，意味着细胞获得噬菌体抗性需要重新调整其生物化学过程。研究表明，通过转录调控三羧酸循环(TCA)、赖氨酸生物合成等关键代谢途径以及细胞内代谢物的含量，细菌代谢过程发生了重组，可能显著影响细胞生理。最后，耐药菌株的SNP分析显示，在研究的假定细菌噬菌体受体中没有氨基酸改变的证据，但在涉及转录调控的基因中检测到一些SNP。这种现象似乎是一种由不同的噬菌体属与细菌相互作用强化的噬菌体特异性的、微调的代谢工程，更新了溶解噬菌体在微生物海洋生态中的作用。
外文摘要：Lytic bacteriophages have been well documented to play a pivotal role in microbial ecology due to their complex interactions with bacterial species, especially in aquatic habitats. Although the use of phages as antimicrobial agents, known as phage therapy, in the aquatic environment has been increasing, recent research has revealed drawbacks due to the development of phage-resistant strains among Gram-negative species. Acquired phage resistance in marine Vibrios has been proven to be a very complicated process utilizing biochemical, metabolic, and molecular adaptation strategies. The results of our multi-omics approach, incorporating transcriptome and metabolome analyses of Vibrio alginolyticus phage-resistant strains, corroborate this prospect. Our results provide insights into phage-tolerant strains diminishing the expression of phage receptors ompF, lamB, and btuB. The same pattern was observed for genes encoding natural nutrient channels, such as rbsA, ptsG, tryP, livH, lysE, and hisp, meaning that the cell needs to readjust its biochemistry to achieve phage resistance. The results showed reprogramming of bacterial metabolism by transcript regulations in key-metabolic pathways, such as the tricarboxylic acid cycle (TCA) and lysine biosynthesis, as well as the content of intracellular metabolites belonging to processes that could also significantly affect the cell physiology. Finally, SNP analysis in resistant strains revealed no evidence of amino acid alterations in the studied putative bacterial phage receptors, but several SNPs were detected in genes involved in transcriptional regulation. This phenomenon appears to be a phage-specific, fine-tuned metabolic engineering, imposed by the different phage genera the bacteria have interacted with, updating the role of lytic phages in microbial marine ecology.
外文关键词：host–；phage interaction；receptors；transporters；Vibrio alginolyticus；acquired phage resistance；metabolic reprogramming；bacteriophages; host metabolism
作者：Skliros, D；Kalatzis, PG；Kalloniati, C；Komaitis, F；Papathanasiou, S；Kouri, ED；Udvardi, MK；Kokkari, C；Katharios, P；Flemetakis, E
作者单位：Agr Univ Athens；Univ Copenhagen；Noble Res Inst；Hellen Ctr Marine Res
期刊名称：VIRUSES-BASEL
期刊影响因子：3.816
出版年份：2021
出版刊次：4
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