中文摘要：尽管对动物脂蛋白致敏机制的某些方面已经有所了解，但它们在很大程度上仍还是谜。研究它们与免疫系统各组成部分的相互作用有可能揭示这种特性。由于这些组成部分非常复杂，本研究拟采用高通量技术进行分析。利用噬菌体展示随机肽库对犬过敏原Can f1进行筛选，鉴定了一个与γδ t-细胞受体抗原结合位点相对应的结合肽。其它的生化研究也证实了这种相互作用。
外文摘要：Although some progress has been achieved in understanding certain aspects of the allergenic mechanism of animal lipocalins, they still remain largely enigmatic. One possibility to unravel this property is to investigate their interaction with components of the immune system. Since these components are highly complex we intended to use a high-throughput technology for this purpose. Therefore, we used phage-display of a random peptide library for panning against the dog allergen Can f 1. By this method we identified a Can f 1 binding peptide corresponding to the antigen-binding site of a putative gamma delta T-cell receptor. Additional biochemical investigations confirmed this interaction.
外文关键词：binding studies；bio-panning；high-throughput assay；mmunogenicity；T-cells
作者：Habeler, M；Redl, B
作者单位：Med Univ Innsbruck
期刊名称：BIOLOGICAL CHEMISTRY
期刊影响因子：3.27
出版年份：2021
出版刊次：4
点击下载：噬菌体展示揭示了脂溶蛋白过敏原Can f1与类似于人γδ t-细胞受体抗原结合区域的肽的相互作用