中文摘要：生物传感器因其在生物检测领域和生物医学诊断中的应用潜力而受到广泛关注，已成为一门新兴学科。生物传感器的发展迫切需要扩展识别元件。通过将噬菌体展示肽库融合到噬菌体外壳蛋白中，使展示肽技术取得了长足进步。噬菌体展示的筛选程序很容易识别各种结合亲和肽，它们是抗体的替代品。噬菌体展示肽的出现弥补了识别分子的不足，在生物传感领域发挥了前所未有的作用。展示肽具有亲和力高、制备成本低、纯度高、适合量产、易于分子水平设计和修饰、对复杂检测环境的耐受性强等优点，近年来作为特异性受体被广泛应用于各种生物传感器。本文综述了噬菌体展示肽在主要生物传感器中的应用前景，并指出了肽传感器的突出性能和不足之处。
外文摘要：Biosensors have been explored for their excellent potential in the field of biological detection and their application in biomedical diagnosis and have become a rapidly emerging subject. The development of biosensors urgently needs support in the expansion of recognition elements. Sequential advancements in displayed peptide technology have been achieved by fusing phage display peptide libraries into phage coat proteins. Screening procedures for phage display readily recognize a variety of binding affinity peptides, which are alternatives to antibodies. The emergence of phage display peptides compensated for the lack of recognition molecules and has played an unprecedented role in the field of biosensing. Displayed peptides have been used for specific receptors in various biosensors in recent years because of advantages such as high affinity, low procedure cost, high purity and mass production, easy design and modification at the molecular level, and increased resistance to the sophisticated detection environment. This review summarizes the feasible applications of phage-displayed peptides in major biosensors and highlights both the outstanding performance and shortcomings of peptide sensors.
外文关键词：Phage display；M13 phage；Peptide；Biosensor；Colorimetric assay； Fluorescence biosensor；Electrochemical sensor；Surface plasmon resonance
作者：Xu, P；Ghosh, S；Gul, AR；Bhamore, JR；Park, JP；Park, TJ
作者单位：Chung Ang Univ
期刊名称：TRAC-TRENDS IN ANALYTICAL CHEMISTRY
期刊影响因子：9.801
出版年份：2021
出版刊次：137
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