中文摘要：众所周知，大肠菌群是水中细菌污染的信息指标。本研究提出了一种使用M13噬菌体修饰还原氧化石墨烯（rGO）的新型化学阻抗生物传感器，用来检测大肠杆菌（ E. coli）。将M13噬菌体作为一种生物识别元件固定在rGO通道上，以便与带负电荷的E.coli结合，使生物传感器产生门控效应，改变其阻抗。用光谱、显微和电学等方法测试了所制备材料和器件的特性。FTIR和XRD测试证明了GO和rGO纳米片制备成功；AFM结果表明所制备的纳米片是单层的。将M13功能化装置浸泡在两种不同浓度的E.coli中，SEM显微图证实了E.coli被成功捕获，并且信号的变化具有浓度依赖性。研究表明，该方法对大肠杆菌具有良好的线性和特异性反应，检出限为45 CFU/mL；此外，该传感器系统对测试大肠菌群还具有选择性。可见，该检测系统具有经济、准确、快速、直接适用的优点，是一种很有前途的大肠菌群检测方法。
外文摘要：Coliform bacteria are well known as informative indicators for bacterial contamination in water. This study presents a novel chemiresistor biosensor using M13 phage-modified reduced graphene oxide (rGO) for detection of Escherichia coli (E. coli), as coliform bacteria. M13 phage, as a biorecognition element, was immobilized on the rGO channel, so that it can bind to negatively charged E. coli bacteria, allowing the gating effect on the biosensor and the change in its resistance. The prepared materials and device were characterized using spectroscopic, microscopic, and electrical measurements. FTIR and XRD results proved the successful fabrication of GO and rGO nanosheets. AFM results showed that the prepared nanosheets were monolayer. The SEM micrographs of the M13-functionalized devices, soaked in two different concentrations of E. coli, confirmed the successful capturing of E. coli and that the signal change is concentration-dependent. As a result, a linear and specific response towards E. coli was observed and the limit of detection was determined to be 45 CFU/mL. Further, the proposed sensor system showed selectivity towards the tested coliforms. These results suggested this sensing system could be a promising tool for detecting coliforms with an economic, accurate, rapid, and directly applicable process.
外文关键词：Phage biosensor；Filed-effect transistor；Graphene；Detection of bacteria；M13-rGO-FET；Electrical sensor
作者：Nakama, K；Sedki, M；Mulchandani, A
作者单位：Univ Calif Riverside；Tokyo Univ Agr & Technol
期刊名称：ANALYTICA CHIMICA ACTA
期刊影响因子：5.977
出版年份：2021
出版刊次：1150
点击下载：用基于还原的氧化石墨烯和M13噬菌体的无标记化学阻抗生物传感器检测大肠菌群