中文摘要：附着在宿主细胞表面并与受体特异性结合是噬菌体感染过程的第一步。裂解噬菌体VP2用于霍乱弧菌生物型El Tor的O1血清群噬菌体分型。但其感染机理知之甚少。本研究旨在鉴定VP2在霍乱弧菌上的受体。发现II型分泌系统（T2SS）中的外膜蛋白EpsD与VP2对霍乱弧菌的特异性吸附有关，而T2SS内膜蛋白EpsM不参与VP2的吸附，但在VP2的成功感染中起作用。VP2的尾丝蛋白gp20与EpsD直接相互作用。研究发现在霍乱弧菌中，除了T2SS作为霍乱毒素分泌和丝状噬菌体释放的运输工具之外，T2SS还可用作噬菌体感染的受体，并可能用作噬菌体DNA注射的通道。本研究丰富了对细菌中T2SS作用的认识。
外文摘要：Attachment and specific binding to the receptor on the host cell surface is the first step in the process of bacteriophage infection. The lytic phage VP2 is used in phage subtyping of the Vibrio cholerae biotype El Tor of the O1 serogroup; however, its infection mechanism is poorly understood. In this study, we aimed to identify its receptor on V. cholerae. The outer membrane protein EpsD in the type II secretory system (T2SS) was found to be related to VP2-specific adsorption to V. cholerae, and the T2SS inner membrane protein EpsM had a role in successful VP2 infection, although it was not related to adsorption of VP2. The tail fiber protein gp20 of VP2 directly interacts with EpsD. Therefore, we found that in V. cholerae, in addition to the roles of the T2SS as the transport apparatus of cholera toxin secretion and filamentous phage release, the T2SS is also used as the receptor for phage infection and probably as the channel for phage DNA injection. Our study expands the understanding of the roles of the T2SS in bacteria.
外文关键词：bacteriophage；type II secretory system；receptor；EpsD；Vibrio cholera
作者：Sun, HH；Liu, M；Fan, FX；Li, Z；Fan, YF；Zhang, JY；Huang, YM；Li, ZP；Li, J；Xu, JL；Kan, BA
作者单位：Chinese Ctr Dis Control & Prevent；Beijing Technol & Business Univ
期刊名称：FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
期刊影响因子：4.123
出版年份：2021
出版刊次：11
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