中文摘要：噬菌体是一种预防和治疗食源性病原体的新型生物控制手段。本研究从废水中分离并鉴定了一种耐碱性大肠杆菌(E. coli)噬菌体，命名为JN01。它对20株大肠杆菌中的16株有裂解活性，其中11株为3重以上耐药菌株。JN01的潜伏期为15min，裂解期为85min。每个受感染细胞的JN01裂解量约为134 PFU。JN01在温度(30°C-70°C)和pH(4-13)等应激条件下相对稳定。JN01的基因组为88360bp的双链DNA分子，共有130个开放阅读框(ORFs)， G+C含量为38.76%。重要的是，在JN01的基因组中没有发现已知的毒力相关、抗生素和溶生相关基因。形态学和系统发育分析证实JN01属于肌病毒科。用JN01在4℃下处理牛奶和生牛肉样品，大肠杆菌O157: H7的活细胞数量减少。可见，JN01可作为食品中大肠杆菌O157: H7的天然抑菌剂。
外文摘要：Bacteriophage is a new biological control tool for the prevention and treatment of foodborne pathogens. In this study, an alkali-resistant phage against Escherichia coli (E. coli), named JN01, was isolated and characterized from wastewater. It exhibited lytic activities against 16 of 20 E. coli strains, 11 of which were more than three multidrug resistant strains. The biological characteristics of JN01 showed that the latent period and lysis period was 15 min and 85 min, respectively. The burst size of JN01 was about 134 PFU per infected cell. JN01 was relatively stable under stressed conditions such as temperatures (30 degrees C-70 degrees C) and pH (4-13). The genome of JN01 is 88,360-bp double-stranded DNA molecules, with a total of 130 open reading frames (ORFs) and 38.76% of G + C content. Importantly, no known virulence-associated, antibiotic and lysogeny-related genes were identified in the genome of JN01. Morphological and phylogenetic analyses confirmed that JN01 belongs to the Myoviridae. Viable cell counts of E. coli O157: H7 were reduced in milk and raw beef samples when treated with JN01 at 4 degrees C. The results indicated that JN01 could be a natural potential biological control agent for E. coli O157: H7 in foods.
外文关键词：Escherichia coli O157:H7；Alkali-resistant bacteriophage；Isolation；Genome；Biological control
作者：Li, YK；Chen, H；Shu, M；Zhong, C；Bi, Y；Yang, HH；Wu, GP
作者单位：Jiangxi Agr Univ
期刊名称：LWT-FOOD SCIENCE AND TECHNOLOGY
期刊影响因子：4.006
出版年份：2021
出版刊次：144
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