中文摘要：噬菌体治疗是一种控制沙门氏菌的可行方法。口服剂型噬菌体微囊化可保护噬菌体免受鸡消化道的破坏。本研究旨在评估沙门氏菌BP FGS011(非囊化和微囊化)经1日龄雏鸡口服后，在体内外条件下通过胃肠道的活性。通过喷雾干燥将噬菌体FGS011封装在含Eudragit(R) L100和Eudragit(R) S100的两种不同ph响应性聚合物中。封装后的噬菌体暴露在前胃-砂囊中，都能在体外条件下存活，而游离的噬菌体则不能。此外，以聚合物Eudragit(R) S100配制的噬菌体更适合于将噬菌体输送到鸡盲肠。在体内试验中，饲喂的游离噬菌体和囊化噬菌体在胃肠道内的浓度无统计学上的显著差异，表明幼雏前胃/砂囊的pH值酸性不够，导致噬菌体滴度差异降低，使游离噬菌体能够在体内存活。
外文摘要：The therapeutic use of bacteriophages is recognized as a viable method to control Salmonella. Microencapsulation of phages in oral dosage forms may protect phages from inherent challenges of the gastrointestinal tract in chickens. Therefore, the main objective of this study was to assess the survival of Salmonella BP FGS011 (non-encapsulated and microencapsulated) through the gastrointestinal tract under in vitro as well as in vivo conditions after oral administration to 1-day-old chicks. To this end, the phage FGS011 was encapsulated in two different pH-responsive formulations with polymers Eudragit(R) L100, and Eudragit(R) S100 using the process of spray drying. Phages encapsulated in either of the two formulations were able to survive exposure to the proventriculus-gizzard in vitro conditions whereas free phages did not. Moreover, phages formulated in polymer Eudragit(R) S100 would be better suited to deliver phage to the caeca in chickens. In the in vivo assay, no statistically significant differences were observed in the phage concentrations across the gastrointestinal tract for either the free phage or the encapsulated phage given to chicks. This suggested that the pH of the proventriculus/gizzard in young chicks is not sufficiently acidic to cause differential phage titre reductions, thereby allowing free phage survival in vivo.
外文关键词：bacteriophages；Salmonella；poultry；Eudragit((R))；encapsulation
作者：Lorenzo-Rebenaque, L；Malik, DJ；Catala-Gregori, P；Marin, C；Sevilla-Navarro, S
作者单位：CEU Univ；Univ Loughborough；Ctr Calidad Avicola & Alimentac Anim Comunidad Va
期刊名称：PHARMACEUTICALS
期刊影响因子：4.286
出版年份：2021
出版刊次：5
点击下载：非囊化和微囊化沙门氏菌噬菌体体内外胃肠道活性研究：家禽噬菌体治疗的意义