中文摘要：利用膜乳化法将大肠杆菌特异性噬菌体包封在三种不同的pH响应性聚合物制剂中，制成100μm的小胶囊，并证明在pH值为1.5时可为噬菌体提供适当的酸保护。所选聚合物制剂可在pH5.5、pH6和pH7下控制噬菌体的释放。还对噬菌体胶囊的其他方面，包括影响包封率的因素、释放动力学、酸稳定性和贮存稳定性进行了评价。本研究将有助于今后评估新的治疗策略，包括允许pH触发噬菌体释放的微生物组编辑方法和将包裹物运送到不同的肠道隔间。胶囊大小的选择是为了便于使用小口径口服灌胃管给药，该管通常在临床前通过小型脊椎动物模型（如小鼠和大鼠）评估药物时使用。
外文摘要：An E.coli-specific phage was encapsulated in three different pH responsive polymer formulations using the process of membrane emulsification. Small 100 mu m capsules were fabricated and shown to afford phages suitable acid protection upon exposure to pH 1.5. Selection of polymer formulations allowed controlled release of phages at pH 5.5, pH 6 and pH 7. Other aspects of phage encapsulation including factors affecting encapsulation yield, release kinetics, acid and storage stability were evaluated. The work presented here would be useful for future evaluation of new therapeutic strategies including microbiome editing approaches allowing pH-triggered release of phages and delivery of encapsulated cargo to different intestinal compartments. The size of the capsules were selected to permit ease of delivery using small bore oral gavage tubes typically used in pre-clinical studies for evaluation of drug substances using small animal vertebrate models such as in mice and rats.
外文关键词：antibiotic resistance；bacteriophages；drug delivery；membrane emulsification；microcapsules；microbiome engineering
作者：Richards, K；Malik, DJ
作者单位：Loughborough Univ
期刊名称：PHARMACEUTICALS
期刊影响因子：4.286
出版年份：2021
出版刊次：5
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