中文摘要：在过去十年中，普遍存在的细菌病毒或噬菌体对地球生物圈产生了巨大影响，杀死每天产生的细菌的4-50%，构成地球上最大的遗传多样性库。目前，与医疗保健服务相关的细菌感染很普遍，而耐抗生素微生物不断激增，直接关系到患者的发病率和死亡率。铜绿假单胞菌在所有医院获得性感染中约占13-15%。该菌是一种机会性病原体，具有代谢多样性和抗生素高(先天性和获得性)耐药性。噬菌体是一种检测细菌感染的高潜力工具，因为这些代谢惰性实体能特异性附着并溶解细菌宿主细胞，从而确认活细胞的存在。本研究从环境源中分离出三种不同的噬菌体，它们具有广泛的溶解谱，能够感染铜绿假单胞菌；能形成清晰而独特的噬菌斑，这是毒性噬菌体独具的显著特征。其次，通过多孔藻酸盐基质、生物聚合物和生物活性显色水凝胶包埋使其结构和功能稳定，将其用作敏感基质，通过与（释放的）细胞质部分的反应产生颜色变化和/或光发射，作为铜绿假单孢菌细胞的生物检测试剂盒。这些噬菌体完整的理化及生物学特性见以往研究论文。
外文摘要：During the last decennium, it has become widely accepted that ubiquitous bacterial viruses, or bacteriophages, exert enormous influences on our planet's biosphere, killing between 4-50% of the daily produced bacteria and constituting the largest genetic diversity pool on our planet. Currently, bacterial infections linked to healthcare services are widespread, which, when associated with the increasing surge of antibiotic-resistant microorganisms, play a major role in patient morbidity and mortality. In this scenario, Pseudomonas aeruginosa alone is responsible for ca. 13-15% of all hospital-acquired infections. The pathogen P. aeruginosa is an opportunistic one, being endowed with metabolic versatility and high (both intrinsic and acquired) resistance to antibiotics. Bacteriophages (or phages) have been recognized as a tool with high potential for the detection of bacterial infections since these metabolically inert entities specifically attach to, and lyse, bacterial host cells, thus, allowing confirmation of the presence of viable cells. In the research effort described herein, three different phages with broad lytic spectrum capable of infecting P. aeruginosa were isolated from environmental sources. The isolated phages were elected on the basis of their ability to form clear and distinctive plaques, which is a hallmark characteristic of virulent phages. Next, their structural and functional stabilization was achieved via entrapment within the matrix of porous alginate, biopolymeric, and bio-reactive, chromogenic hydrogels aiming at their use as sensitive matrices producing both color changes and/or light emissions evolving from a reaction with (released) cytoplasmic moieties, as a bio-detection kit for P. aeruginosa cells. Full physicochemical and biological characterization of the isolated bacteriophages was the subject of a previous research paper.
外文关键词：bacteriophage particles；Pseudomonas aeruginosa；immobilization and structural；functional stabilization；bacterial biosensing；bio-reactive polymeric matrix；chromogenic；bioluminescent bio-hydrogel
作者：Harada, LK；Junior, WB；Silva, EC；Oliveira, TJ；Moreli, FC；Junior, JMO；Tubino, M；Vila, MMDC；Balcao, VM
作者单位：Univ Sorocaba；Fac Technol Sorocaba FATEC SO；Univ Estadual Campinas；Univ Aveiro
期刊名称：BIOSENSORS-BASEL
期刊影响因子：3.24
出版年份：2021
出版刊次：4
点击下载：基于噬菌体的铜绿假单胞菌生物传感:一种公认的实时检测多重耐药菌株的综合方法