中文摘要：临床上认为噬菌体细胞裂解酶是目前最先进的一类抗菌药物，具有降解肽聚糖的新型作用模式。细胞裂解酶最初因革兰氏阴性病原体外膜不透水而被排除用来对抗该菌，但现在正日益发展成为这些关键优先病原体的有效抗菌剂。总的来说，最近探索三条研究路线并取得了不同程度的进展，包括使用由于带有正电荷的c端破坏了外膜的稳定性而具有内在活性的细胞裂解酶，利用物理或化学手段破坏外膜的完整性和蛋白质工程，使细胞裂解酶配备必要的工具来克服外膜。
外文摘要：Phage lysins are one of the most advanced classes of antibacterials under clinical evaluation and have a new mode of action based on peptidoglycan degradation. Lysins were initially excluded from use against Gram-negative pathogens because of their impermeable outer membrane, but are now increasingly developed as effective antibacterials against these critical priority pathogens. Generally, three routes of investigation have been recently explored and advanced to different extents, including the use of lysins that possess intrinsic activity due to a positively charged C-terminus that destabilizes the outer membrane, the use of physical or chemical means to disrupt the outer membrane integrity and protein engineering to equip the lysin with the necessary tools to overcome the outer membrane.
外文关键词：ESCHERICHIA-COLI；PHAGE LYSIN；ACINETOBACTER-BAUMANNII； PSEUDOMONAS-AERUGINOSA；ANTIBACTERIAL ACTIVITY；RESISTANT；ENDOLYSIN； ART-175PERMEABILITY；PEPTIDES
作者：Gutierrez, D；Briers, Y
作者单位：Univ Ghent
期刊名称：CURRENT OPINION IN BIOTECHNOLOGY
期刊影响因子：8.288
出版年份：2021
出版刊次：S1
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