中文摘要：噬菌体疗法，即临床用来杀死细菌的病毒，是对抗抗菌素耐药性的一种有前途的策略。给药前，要仔细检查噬菌体的安全性及其与目标细菌的相互作用，但很少涉及到识别细菌表面参与噬菌体吸附的受体。本文提出，了解这些噬菌体受体的功能和位置，可以打开改进和创新使用噬菌体治疗的新大门。有了噬菌体受体的知识，就可以设计智能的噬菌体混合物，发现新的噬菌体来源的抗菌素，并引导噬菌体耐药性朝着临床可利用的表型进化。为了启动这一计划，建议优先考虑宿主和噬菌体。最后，对噬菌体受体的鉴定方法进行了展望，包括高通量诱变分子平台、合成生物学和机器学习。
外文摘要：Phage therapy, the clinical use of viruses that kill bacteria, is a promising strategy in the fight against antimicrobial resistance. Before administration, phages under go a careful examination of their safety and interactions with target bacteria. This characterization seldom includes identifying the receptor on the bacterial surface involved in phage adsorption. In this perspective article, we propose that understanding the function and location of these phage receptors can open the door to improved and innovative ways to use phage therapy. With knowledge of phage receptors, we can design intelligent phage cocktails, discover new phage-derived antimicrobials, and steer the evolution of phage-resistance towards clinically exploitable phenotypes. In an effort to jump-start this initiative, we recommend priority groups of hosts and phages. Finally, were view modern approaches for the identification of phage receptors, including molecular platforms for high-throughput mutagenesis, synthetic biology, and machine learning.
外文关键词：HOST-RANGE；RESISTANCE；TWORT
作者：Altamirano, FLG；Barr, JJ
作者单位：Monash Univ
期刊名称：CURRENT OPINION IN BIOTECHNOLOGY
期刊影响因子：8.288
出版年份：2021
出版刊次：SI
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