中文摘要：噬菌体在感染过程中利用专门的尾部机械将蛋白质和遗传物质输送到细菌细胞中。附着在它们尾部末端的是受体结合蛋白（RBP），能识别暴露在宿主细菌表面的特定分子。由于自然产生的噬菌体的治疗能力往往受到狭窄的宿主范围的限制，因此通过定向进化或结构导向的RBP工程来扩大其宿主范围成为研究的热点。本文描述了不同RBP工程平台的设计原则，并关注RBP结合的机制以及噬菌体在细菌表面的正确时空附着。深入了解这些机制将直接有助于今后更有效的噬菌体治疗工程。
外文摘要：Bacteriophages (phages) use specialized tail machinery to deliver proteins and genetic material into a bacterial cell during infection. Attached at the distal ends of their tails are receptor binding proteins (RBPs) that recognize specific molecules exposed on host bacteria surfaces. Since the therapeutic capacity of naturally occurring phages is often limited by narrow host ranges, there is significant interest in expanding their host range via directed evolution or structure-guided engineering of their RBPs. Here, we describe the design principles of different RBP engineering platforms and draw attention to the mechanisms linking RBP binding and the correct spatial and temporal attachment of the phage to the bacterial surface. A deeper understanding of these mechanisms will directly benefit future engineering of more effective phage-based therapeutics.
外文关键词：CRISPR-CAS；CRYSTAL-STRUCTURE；TAIL SPIKE；K5 LYASE；T2 PHAGE；TAILSPIKE；INFECTION；DOMAIN；T4；ENDOSIALIDASE
作者：Dunne, M；Prokhorov, NS；Loessner, MJ；Leiman, PG
作者单位：Swiss Fed Inst Technol；Univ Texas Med Branch
期刊名称：CURRENT OPINION IN BIOTECHNOLOGY
期刊影响因子：8.288
出版年份：2021
出版刊次：SI
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