中文摘要：单链(ss)DNA纳米结构(DNs)由千碱基ssDNA单分子折叠而成，是一种适用于生物材料和生物医学应用的新型DNA结构。本文使用DNA切割DNA酶（脱氧核酶），提出了一个高效、经济地生产毫克级单链ssDN的方法。设计了以自裂解脱氧核酶为侧翼的ssDN基因，并以单链形式进行扩增，产生的扩增子释放出万亿自我折叠成所需结构的ssDNA拷贝。通过设计反式作用脱氧核酶，证实了几种不同形状的ssDN可以进行编码和扩增；与从扩增子中特定形状的可控释放相结合，提供了一种方便而经济的方法，将纳米结构的“遗传密码”安全地储存在DNA上，以便按需进行选择性大规模生产。本文证明了ssDN辅助脂质体分选的能力，并期望该方法能促进ssDN在广泛领域的应用。
外文摘要：Single-stranded (ss) DNA nanostructures (DNs), created by unimolecular folding of kilo-based ssDNA, represent a new class of DNA constructs suitable for biomaterial and biomedical applications. Here, using DNA-cleaving DNA enzymes (deoxyribozymes), we present a strategy for the efficient and cost-effective production of milligrams of ssDNs. We designed "genes'' of ssDNs flanked by self-cleaving deoxyribozymes and amplified the genes in the single-stranded form. Upon autoprocessing, the resulting amplicons release a quadrillion copies of the ssDNA that self-folds into the desired structures. By engineering trans-acting deoxyribozymes, we showed that several ssDNs of different shapes can be encoded and amplified in tandem with controllable release of specific shapes from the amplicons, thereby providing a convenient yet economical way to safely store "genetic codes'' of nanostructures on DNA for selective mass production on demand. We demonstrated ssDNs' ability to assist liposome sorting and expect the approach will promote the development of ssDNs' applications in broad areas.
外文关键词：ORIGAMI NANOSTRUCTURES；CELLULAR UPTAKE；PHAGE DNA；AMPLIFICATION； REPLICATION；POLYMERASE；STABILITY；PLASMID；COMPLEX；ENTRY
作者：Jia, YL；Chen, LM；Liu, J；Li, W；Gu, HZ
作者单位：Fudan Univ
期刊名称：CHEM
期刊影响因子：19.735
出版年份：2021
出版刊次：4
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