中文摘要：爆炸物、毒品、环境激素和变质食品等诸多威胁随着其挥发性有机化合物（VOCs）呈现在我们周围。为了尽早识别和应对这些威胁，需要高灵敏度和高选择性的电子鼻。基于M13噬菌体的光电鼻可以满足这些要求，但其研究尚处于起步阶段，系统方法和开发策略至关重要。本文整合了理论和实验方法，分析了基因工程噬菌体表面化学与噬菌体光电鼻特性之间的相关性。定量分析了基因工程噬菌体色膜对部分VOCs的反应，并与密度泛函理论(DFT)计算的结合亲和值进行相关性比较。研究表明噬菌体色膜通过基因工程具有可控的反应性。采用层次聚类分析(HCA)法，选取了有利于区分各VOCs的噬菌体。通过DFT计算优化的几何结构验证了这种差异的原因。并证实了基于色氨酸和组氨酸的基因工程噬菌体膜，对鉴别用于评估桃新鲜度的挥发性有机化合物（Y-己内酯、2-异丙基-4-甲基噻唑、乙醇、丙酮、乙酸乙酯和乙醛）具有重要意义。将该方法应用于设计田间应用的噬菌体光电鼻，并通过测定实际水果新鲜度加以验证。
外文摘要：Various threats such as explosives, drugs, environmental hormones, and spoiled food manifest themselves with the presence of volatile organic compounds (VOCs) in our environment. In order to recognize and respond to these threats early, the demand for highly sensitive and selective electronic noses is increasing. The M13 bacteriophage-based optoelectronic nose is an excellent candidate to meet all these requirements. However, the phage-based electronic nose is still in its infancy, and strategies that include a systematic approach and development are still essential. Here, we have integrated theoretical and experimental approaches to analyze the correlation between the surface chemistry of genetically engineered phage and the phage-based optoelectronic nose properties. The reactivity of the genetically engineered phage color film to some VOCs were quantitatively analyzed, and the correlation with the binding affinity value calculated by Density-functional theory (DFT) was compared. This demonstrates that phage color films have controllable reactivity through a genetic engineering. We have selected phages that are advantageous in distinguishing each VOCs in this work through hierarchical cluster analysis (HCA). The reason for this difference was verified through the optimized geometry calculated by DFT. Through this, it was confirmed that the tryptophan-based and the Histidine-based of genetically engineered phage film are important in distinguishing the VOCs (Y-hexanolactone, 2-isopropyl-4-methylthiazole, ethanol, acetone, ethyl acetate, and acetaldehyde) used in this work to evaluate the peach freshness quality. This was applied to the design of a field-applied phage-based optoelectronic nose and verified by measuring the freshness of the actual fruit.
外文关键词：Bioelectronic nose；Bacteriophage；Genetic engineering；Biomaterials； Surface chemistry
作者：Lee, JM；Lee, Y；Devaraj, V；Nguyen, TM；Kim, YJ；Kim, YH；Kim, C；Choi, EJ；Han, DW；Oh, JW
作者单位：Pusan Natl Univ
期刊名称：BIOSENSORS & BIOELECTRONICS
期刊影响因子：10.257
出版年份：2021
出版刊次：188
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