中文摘要：跨越物理屏障的分子通讯需要孔隙连接两边的环境并区分扩散剂。本研究使用噬菌体P22衍生的多孔病毒样颗粒（VLPs），研究能够进入其内部的分子大小范围。尽管有VLP的低温电磁模型，但由于溶液中P22粒子的动态特性，无法准确地描述能够穿过孔隙的分子参数。将AdhD酶包埋在P22 VLP中，使用PAMAM树状大分子修饰NADH/NAD+的氧化还原反应，研究进入P22的分子大小和电荷限制。利用P22粒子的三种不同的可接近形态，确定了每种粒子的有效孔径，并证明带负电荷的基质，尽管外部带负电荷，却比中性基质更容易扩散。通过物理屏障运输分子需要与环境相连的孔隙。本研究使用封装的氧化还原系统，报道了一项基于溶液的动态研究，研究了大小和电荷对通过噬菌体P22衍生的病毒样颗粒孔道运输的影响。
外文摘要：Molecular communication across physical barriers requires pores to connect the environments on either side and discriminate between the diffusants. Here we use porous virus-like particles (VLPs) derived from bacteriophage P22 to investigate the range of molecule sizes able to gain access to its interior. Although there are cryo-EM models of the VLP, they may not accurately depict the parameters of the molecules able to pass across the pores due to the dynamic nature of the P22 particles in the solution. After encapsulating the enzyme AdhD within the P22 VLPs, we use a redox reaction involving PAMAM dendrimer modified NADH/NAD+ to examine the size and charge limitations of molecules entering P22. Utilizing the three different accessible morphologies of the P22 particles, we determine the effective pore sizes of each and demonstrate that negatively charged substrates diffuse across more readily when compared to those that are neutral, despite the negatively charge exterior of the particles. Molecular transport across physical barriers requires pores that connect to the environment. Here, the authors report on a solution based dynamic study into the effects of size and charge on the transport through the pores of virus-like particles derived from bacteriophage P22 using an encapsulated redox system.
外文关键词：ENZYME ENCAPSULATION；PORE DIMENSIONS； COAT PROTEIN；MECHANISM；MATURATION；RELEASE；SITE；NAD
作者：Selivanovitch, E；LaFrance, B；Douglas, T
作者单位：Indiana Univ；Univ Calif Berkeley
期刊名称：NATURE COMMUNICATIONS
期刊影响因子：12.121
出版年份：2021
出版刊次：1
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