中文摘要：最近FDA批准了基于美蓝(MB)的光动力疗法(PDT)用于治疗人类各种疾病，引起了人们的极大兴趣。本文首次系统评价了NIR控制下，游离MB和附着在NaYF4:Yb, Er纳米颗粒(UCNPs)中的MB如MB@UCNPs，封装在脂质体(LPs)中的PDT效率。UCNPs作为光敏剂染料MB的上转换能量源，增强ROS的产生，剩余能量可用于选择性跟踪或生物成像。通过噬菌体展示技术结合新发现的抗HER-2肽，实现该纳米LPs系统的特异性，并通过体外HER-2过度表达模型共焦成像得到了证实。在975nm(0.60W/cm(-2))NIR激光连续照射5min后，含MB@UCNPs的LPs和含UCNPs的游离MB的LPs，细胞活力分别下降了83%和64%。此外，流式细胞术检测证实，MB@UCNPs的LPs增强ROS生成能力高于UCNPs的游离MB，说明了MB和UCNPs之间的最小距离与抗癌效率相关。
外文摘要：Methylene Blue (MB) based Photodynamic therapy (PDT) has gained much interest recently due to its FDA approval for treating various diseased conditions in human. Herein, we report for the first time the systematic evaluation of NIR-controlled PDT efficiency of free MB and MB attached on NaYF4:Yb, Er nanoparticles (UCNPs) i.e., MB@UCNPs encapsulated within Liposomes (LPs). The UCNPs act as an upconverting energy source for the photosensitizers dye MB for enhanced ROS generation and utilizing the remnant energy for selective tracking or bioimaging. The specificity of our nano LPs system was achieved by conjugating our newly discovered anti-HER2 peptides by phage display technique and demonstrated its specificity by confocal imaging of HER-2 overexpressing in vitro model. A significant decline in the cell viability by 83% and 64% was achieved by the LPs containing MB@UCNPs and free MB with UCNPs, respectively after 975 nm NIR laser exposure (0.60 W/cm(-2)) for 5 min continuous exposure. Also, the flowcytometry assay confirmed that enhanced ROS generation capability of LPs with MB@UCNPs was higher than the free MB with UCNPs, which states the relevance of the minimal distance between MB and UCNPs for higher anti-cancer efficiency.
外文关键词：Liposome；Photodynamic therapy (PDT)；Upconversion bioimaging；Lipid nanoparticle；Methylene blue dye；Anti-HER2 peptide；Theranostics
作者：Panikar, SS；Ramirez-Garcia, G；Banu, N；Vallejo-Cardona, AA；Lugo-Fabres, P；Camacho-Villegas, TA；Salas, P；de la Rosa, E
作者单位：Univ De La Salle Bajio；Univ Nacl Autonoma Mexico；Univ Guadalajara；Ctr Invest & Asistencia Technol & Diseno Estado J
期刊名称：JOURNAL OF LUMINESCENCE
期刊影响因子：3.28
出版年份：2021
出版刊次：237
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