中文摘要：微囊藻毒素是一组由存在于地表水中的蓝藻细菌产生的肝毒素。本研究研发了两种猝灭体(Q -体)作为有效可靠的荧光传感器，可在3分钟内检测到含有亮氨酸和精氨酸的微囊藻毒素(MC-LR)。利用设计的抗体3A8单链可变区，将荧光染料ATTO520或四甲基罗丹明（TAMRA）偶联到其N端，制备Q-体。抗体内色氨酸残基通过光诱导电子转移使Q-体荧光猝灭，而与MC-LR结合后Q-体荧光显著增强。TMARA标记的Q-体，检出限为0.45ng/mL，EC50为20.43ng/mL；ATTO520标记的Q-体的检出限为8.6ng/mL，EC50为251.6ng/mL。结合Q-体可以实现MC-LR的灵敏和宽范围检测。添加MC-LR的河水样品回收率为94.8%-120.0%，表明其在自然界MC-LR监测中具有潜在的应用价值。
外文摘要：Microcystins are a group of hepatotoxins produced by cyanobacteria present in surface water. We developed two Quenbodies (Q-bodies) as effective and reliable fluorescent sensors to detect microcystin with leucine and arginine (MC-LR) within 3 min. The designed single-chain variable region of antibody 3A8 was used to prepare Q-bodies by conjugating the fluorescent dye ATTO520 or tetramethylrhodamine (TAMRA) to its N-terminus. The fluorescence of Q-bodies was quenched by the internal tryptophan residues of antibody via photo-induced electron transfer, whereas it significantly increased upon the binding of MC-LR. The limit of detection and EC50 were 0.45 ng/mL and 20.43 ng/mL, respectively, for TMARA-labeled Q-body, and 8.6 ng/mL and 251.6 ng/ mL for ATTO520-labeled Q-body. Combining the Q-bodies enabled sensitive and wide-range detection of MC-LR. The recovery rates for river water samples spiked with MC-LR ranged from 94.8% to 120.0%, suggesting their potential applications in MC-LR monitoring in nature.
外文关键词：Microcystin-LR；Immunosensor；Quenchbody；Fluorescence；Rapid detection
作者：Zhang, LQ；Dong, H；Li, HM；Li, BW；Zhao, GW；Cai, HL；Chen, LM；Dong, JH
作者单位：Weifang Med Univ；Peking Univ；Fangzi Ctr Dis Control & Prevent；Tokyo Inst Technol
期刊名称：MICROCHEMICAL JOURNAL
期刊影响因子：3.954
出版年份：2021
出版刊次：167
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