中文摘要：硝基芳香族爆炸物的检测已引起世界各国对人体安全的关注。本研究介绍了一种负载bioreporter的生物相容性多孔微球荧光生物传感器，用于检测硝基芳香族化合物。以嵌入细菌bioreporter的乳酸-乙醇酸共聚物为载体，设计了一种新型的生物传感器。基因工程细菌bioreporter可以表达一种绿色荧光蛋白，响应硝基芳香化合物（如三硝基甲苯和二硝基甲苯）。改性微球的表面结构提供了较大的表面积，并且易于渗透，增加了附着的bioreporter数量，增强了生物传感器的荧光信号。此外，在开放多孔微球中加入M13噬菌体，可以通过M13噬菌体肽段与硝基芳香族化合物间pi-pi相互作用，显著放大检测荧光信号。表面形态的改变以及M13噬菌体的基因改造，大大增强了荧光信号，使炸药的检测更加容易，在野外掩埋TNT的防区外遥感方面具有很大潜力。
外文摘要：The detection of explosive nitroaromatic compounds has caused worldwide concern for human safety. In this study, we introduce a fluorescent biosensor based on porous biocompatible microspheres loaded with a bioreporter for the detection of nitroaromatic compounds. Poly(lactic-co-glycolic acid) microbeads were designed as biosensors embedded with the bacterial bioreporters. The genetically engineered bacterial bioreporter can express a green fluorescent protein in response to nitroaromatic compounds (e.g., trinitrotoluene and dinitrotoluene). The modified surface structure in microbeads provides a large surface area, as well as easy penetration, and increases the number of attached bioreporters for enhanced fluorescent signals of biosensors. Moreover, the addition of the M13 bacteriophage in open porous microbeads significantly amplified the fluorescence signal for detection by the pi-pi interaction between peptides in the M13 bacteriophage and nitroaromatic compounds. The modification of the surface morphology, as well as the genetically engineered M13 phage, significantly amplifies the fluorescence signal, which makes the detection of explosives easier, and has great potential for the stand-off remote sensing of TNT buried in the field.
外文关键词：EXPLOSIVES DETECTION；GAS-CHROMATOGRAPHY；SURFACE；NANOPARTICLES；SPECTROMETRY；ADHESION；SENSOR；CELLS；PLGA
作者：EXPLOSIVES DETECTION；GAS-CHROMATOGRAPHY；SURFACE；NANOPARTICLES；SPECTROMETRY；ADHESION；SENSOR；CELLS；PLGA
作者单位：Kookmin Univ；Sejong Univ
期刊名称：ANALYST
期刊影响因子：3.978
出版年份：2021
出版刊次：
点击下载：利用嵌在多孔乳酸-乙醇酸共聚微球中的细菌进行硝基芳香族化合物的增强荧光检测