中文摘要：溶藻弧菌是感染人和多种水生动物的重要病原菌之一，可引起人食物中毒和败血症。广泛使用的抗生素正逐渐失去效用，而噬菌体作为一种新的抗菌策略，正受到越来越多的关注。为了更好地控制致病菌，本研究从海鲜市场的污水中分离了一种新型溶藻弧菌噬菌体BUCT549。经透射电镜观察和系统发育树分析，该病毒为长尾病毒科新成员。本文依据VIRIDIC计算的基因组间相似性(最大为56%)，建议建立一个新的属BUCT549。噬菌体BUCT549在较宽的pH（3.0-11.0）范围和高温（高达60℃）环境中具有稳定性，可用于噬菌体治疗。潜伏期30-40min，细菌爆发量为141 PFU/感染。在基于末端酶大亚基的系统发育树中，BUCT549与8种不同宿主的弧菌噬菌体密切相关。同时，实验证明BUCT549具有感染副溶血性弧菌的能力。通过协同进化实验确定，三株耐受溶藻弧菌通过MSHA相关膜蛋白表达基因突变来逃避噬菌体侵染，导致鞭毛丢失。本文对新型噬菌体的识别和侵染机制的研究，将有助于噬菌体成为生物防治制剂的重要组成部分。
外文摘要：Vibrio alginolyticus is one of the most important of pathogens that can infect humans and a variety of aquatic animals, and it can cause food poisoning and septicemia in humans. Widely used antibiotics are gradually losing their usefulness, and phages are gaining more attention as new antibacterial strategies. To have more potential strategies for controlling pathogenic bacteria, we isolated a novel V. alginolyticus phage BUCT549 from seafood market sewage. It was classified as a new member of the family Siphoviridae by transmission electron microscopy and a phylogenetic tree. We propose creating a new genus for BUCT549 based on the intergenomic similarities (maximum is 56%) obtained from VIRIDIC calculations. Phage BUCT549 could be used for phage therapy due to its stability in a wide pH (3.0-11.0) range and high-temperature (up to 60 degrees C) environment. It had a latent period of 30-40 min and a burst size of 141 PFU/infected bacterium. In the phylogenetic tree based on a terminase large subunit, BUCT549 was closely related to eight Vibrio phages with different species of host. Meanwhile, our experiments proved that BUCT549 has the ability to infect a strain of Vibrio parahaemolyticus. A coevolution experiment determined that three strains of tolerant V. alginolyticus evaded phage infestation by mutating the MSHA-related membrane protein expression genes, which caused the loss of flagellum. This research on novel phage identification and the mechanism of infestation will help phages to become an integral part of the strategy for biological control agents.
外文关键词：vibrio alginolyticus；bacteriophage；receptor；MSHA protein；genome analysis
作者：Li, J；Tian, FJ；Hu, YJ；Lin, W；Liu, YJ；Zhao, FY；Ren, HY；Pan, Q；Shi, TX；Tong, YG
作者单位：Beijing Univ Chem Technol；Qingdao Phagepharm Biotech Co Ltd；Acad Mil Med Sci
期刊名称：FRONTIERS IN MICROBIOLOGY
期刊影响因子：5.085
出版年份：2021
出版刊次：12
点击下载：以鞭毛为受体的新型溶藻弧菌噬菌体BUCT549的特性及基因组分析