中文摘要：医院或医院获得性感染(HAIs)对全球死亡率有重大影响。肠球菌和葡萄球菌是引起HAIs的主要原因之一，加上它们对抗生素的耐药性增加，因此成为需要重点控制的病原体。HAIs的金标准诊断方法费时费力，不利于及时、充分的治疗。因此，当务之急是开发快速、准确的诊断工具。本研究结合磁阻(MR)传感器的灵敏度、实验室芯片平台的便携性及噬菌体受体结合蛋白(RBPs)的特异性作为探针，快速、多重检测肠球菌和葡萄球菌。为此，首先用RBPs功能化的磁性纳米粒子（MNPs）标记细菌细胞，然后通过MR传感器进行测量。结果表明，RBP-MNPS可单个和同时特异性捕获70%以上的肠球菌和葡萄球菌细胞。此外，MR传感器在2小时内检测出样品中两种低浓度（10 CFU/ml）的病原体，信号很强。总之，该实验室芯片MR平台具有很大的潜力，可用于快速、灵敏和特异的细菌感染多重诊断。
外文摘要：Nosocomial or hospital-acquired infections (HAIs) have a major impact on mortality worldwide. Enterococcus and Staphylococcus are among the leading causes of HAIs and thus are important pathogens to control mainly due to their increased antibiotic resistance. The gold-standard diagnostic methods for HAIs are time-consuming, which hinders timely and adequate treatment. Therefore, the development of fast and accurate diagnostic tools is an urgent demand. In this study, we combined the sensitivity of magnetoresistive (MR) sensors, the portability of a lab-on-chip platform, and the specificity of phage receptor binding proteins (RBPs) as probes for the rapid and multiplex detection of Enterococcus and Staphylococcus. For this, bacterial cells were firstly labelled with magnetic nanoparticles (MNPs) functionalized with RBPs and then measured on the MR sensors. The results indicate that the RBP-MNPS provided a specific individual and simultaneous capture of more than 70% of Enterococcus and Staphylococcus cells. Moreover, high signals from the MR sensors were obtained for these samples, providing the detection of both pathogens at low concentrations (10 CFU/ml) in less than 2 h. Overall, the lab-on-chip MR platform herein presented holds great potential to be used as a point-of-care for the rapid, sensitive and specific multiplex diagnosis of bacterial infections.
外文关键词：bacteriophages；magnetic nanoparticles；magnetoresistive sensors；nosocomial infections；receptor binding proteins
作者：Cunha, AP；Henriques, R；Cardoso, S；Freitas, PP；Carvalho, CM
作者单位：Int Iberian Nanotechnol Lab INL；Univ Minho；Inst Engn Sistemas & Computadores Microsistemas &；IN Inst Nanosci & Nanotechnol
期刊名称：BIOTECHNOLOGY AND BIOENGINEERING
期刊影响因子：4.002
出版年份：2021
出版刊次：
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