中文摘要：食源性致病菌已造成许多公共卫生事件和沉重的经济负担。已经证实细胞内溶素能有效杀灭病原菌，且耐药发生率低。本研究通过原核表达获得了鼠伤寒沙门氏菌裂解噬菌体SLMP1编码的重组细胞内溶素LysSP1，并分析了其特性。发现乙二胺四乙酸（EDTA）可作为外膜通透剂，提高LysSP1的杀菌活性；在5mmol/L EDTA的协同作用下，LysSP1对鼠伤寒沙门菌ATCC14028具有较强的杀菌活性；LysSP1在4℃下稳定7天，在-20℃下稳定180天；LysSP1在40℃时仍能保持最佳活性，在碱性条件（ph8.0-10.0）下活性较好；二价金属离子不能提高LysSP1的杀菌活性，反而导致杀菌活性显著降低；LysSP1不仅能裂解沙门氏菌，还能裂解其它革兰氏阴性菌和革兰氏阳性菌。可见，LysSP1是一种广谱的内溶蛋白，具有抗沙门氏菌和其他食源性病原体的潜力。
外文摘要：Foodborne pathogens have caused many public health incidents and heavy economic burden. Endolysins have been proven to have efficient bactericidal activity against pathogens with low incidence of resistance. In this study, the recombinant endolysin LysSP1 encoded by Salmonella Typhimurium lytic bacteriophage SLMP1 was obtained by prokaryotic expression, and its characteristics were analyzed. Ethylenediaminetetraacetic acid (EDTA) can be used as the outer membrane permeabilizer to increase the bactericidal activity of LysSP1. Under the synergism of 5 mmol/L EDTA, LysSP1 exhibited a strong bactericidal activity against Salmonella Typhimurium ATCC14028. LysSP1 was stable at 4 degrees C for 7 days and at -20 degrees C for 180 days. LysSP1 remained the optimal activity at 40 degrees C and was efficiently active at alkaline condition (pH 8.0-10.0). Divalent metal ions could not enhance the bactericidal activity of LysSP1 and even caused the significant reduction of bactericidal activity. LysSP1 not only could lyse Salmonella, but also could lyse other Gram-negative strains and Gram-positive strains. These results indicated that LysSP1 is a broad-spectrum endolysin and has potential as an antimicrobial agent against Salmonella and other foodborne pathogens.
外文关键词：Bacteriophage；Endolysin；Salmonella；Bactericidal activity
作者：Jiang, YH；Xu, DQ；Wang, LZ；Qu, M；Li, FL；Tan, ZJ；Yao, L
作者单位：Chinese Acad Fishery Sci；Shanghai Ocean Univ；Pilot Natl Lab Marine Sci & Technol
期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
期刊影响因子：3.53
出版年份：2021
出版刊次：
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