中文摘要：目前，生物材料已用于药物的控释，水凝胶是治疗浅表和深部伤口的敷料成分。本研究旨在将噬菌体（抗菌剂）加入聚乙烯醇（PVA）水凝胶中，用于治疗皮肤感染。将噬菌体（UFV-AREG1）掺入PVA中，以PVA为对照，进行溶胀试验、傅立叶变换红外光谱（FTIR）、DSC和TGA热分析以及对大肠杆菌O157:H7的抑菌作用。发现PVA-噬菌体膜的溶胀率大于PVA-对照膜，表明PVA-噬菌体有更多的水分子扩散到聚合物网络o中，促进噬菌体释放到水介质中；PVA-噬菌体抑制面积为3.715cm2，对照组为2.916cm2，标准差为128.7cm2，PVA-噬菌体抑制区明显高于-PVA对照组，与液体培养基中由酶标仪测得的微生物生长曲线表示的抗菌分析结果一致；FTIR分析表明，PVA-噬菌体在2860和2930cm-1之间有一个峰值，而PVA-对照则没有，可能与添加的噬菌体有关；热分析表明，二者之间没有显著差异。可见，将噬菌体加入PVA水凝胶中，并评估其释放情况，可用于治疗皮肤感染。
外文摘要：Currently, biomaterials have been used for the controlled release of drugs and hydrogels have been indicated as dressing components to treat superficial and deep wounds. The objective of this work, thus, was to incorporate the bacteriophage (antimicrobial) in polyvinyl alcohol (PVA) hydrogel for future application in skin infections. The bacteriophage (UFV-AREG1) was incorporated in PVA and (PVA-control and PVA-phage) were submitted to Swelling tests, Fourier Transform Infrared Spectroscopy (FTIR), DSC and TGA Thermal Analysis and antimicrobial effect on E. coli O157:H7. Swelling of the PVA-phage film (38%) was greater than the PVA-control (15%), which indicates in the PVA-phage (p < 0.05) a greater diffusion of water molecules into the polymeric network o which facilitates the release of the phage into the aqueous medium. The PVA-phage inhibition area was 3.715 cm2 versus 2.916 cm2 of the control with a standard deviation of 128.7 cm2. The PVA-phage inhibition zone was more significant than the PVA-control (p < 0.05). These results corroborate with antimicrobial analysis in liquid medium represented by the microbial growth curve obtained by the microplate reader. FTIR analyzes showed a peak for PVA-phage between the 2860 and 2930 cm-1 bands not found in the PVA-control and may be related to the added phage. Thermal analysis showed no significant differences between hydrogels. Therefore, it was possible to incorporate the bacteriophage in a PVA hydrogel and evaluate its release for later application to the treatment of skin infections.
外文关键词：Polyvinyl alcohol hydrogel；Phage therapy；Bacterial infections；Curative adhesive
作者：Boggione, DMG；Santos, IJB；de Souza, SM；Mendonca, RCS
作者单位：Univ Fed Vicosa；Univ Fed Sao Joao del Rei
期刊名称：JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
期刊影响因子：2.734
出版年份：2021
出版刊次：63
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