中文摘要：食源性疾病的爆发仍是公共卫生和食品安全的一个主要问题。及时检测可以确保零售前食品安全，从而保护消费者免受食源性疾病的危害。这就需要大量快速、灵敏、准确的检测工具。近年来人们一直在探索各种有效方法，将抗体、寡核苷酸、噬菌体和哺乳动物细胞作为生物传感器平台的信号转导和分析物识别探针，以期实现高特异性和低检测限。哺乳动物细胞型和噬菌体型传感器的发展已经产生了能够检测低水平病原体和区分活细胞和死细胞的传感器。生物技术平台的组合增加了生物传感器检测食源性病原体的实用性和应用性。然而，还需要对具有复杂食物基质的生物传感器进行进一步的严格测试，以确保这些传感器用于医疗点需求和疫情调查。
重点
生物传感器可用于各种食物基质中的病原体检测。
生物传感器的特异性和灵敏性依赖于生物识别分子和信号输出。
噬菌体传感器具有高度特异性，其使用越来越普遍。
细胞传感器可以确定病原体和毒素的毒力。
外文摘要：Foodborne disease outbreaks continue to be a major public health and food safety concern. Testing products promptly can protect consumers from foodborne diseases by ensuring the safety of food before retail distribution. Fast, sensitive, and accurate detection tools are in great demand. Therefore, various approaches have been explored recently to find a more effective way to incorporate antibodies, oligonucleotides, phages, and mammalian cells as signal transducers and analyte recognition probes on biosensor platforms. The ultimate goal is to achieve high specificity and low detection limits (1 to 100 bacterial cells or piconanogram concentrations of toxins). Advancements in mammalian cell-based and bacteriophage-based sensors have produced sensors that detect low levels of pathogens and differentiate live from dead cells. Combinations of biotechnology platforms have increased the practical utility and application of biosensors for detection of foodborne pathogens. However, further rigorous testing of biosensors with complex food matrices is needed to ensure the utility of these sensors for point-ofcare needs and outbreak investigations.
   HIGHLIGHTS
Biosensors can be used for pathogen detection in various food matrices.
Specificity and sensitivity of biosensors rely on biorecognition molecules and signal output.
Working principles of different biosensors can be combined for improved sensitivity.
Bacteriophage-based sensors are highly specific and becoming more commonly used. Cell-based sensors can determine the virulence of viable and active pathogens and toxins.
外文关键词：Biosensor；Escherichia coli；Foodborne pathogens；Food safety；Listeria；Salmonella
作者：Xu, LP；Bai, XJ；Bhunia, AK
作者单位：Purdue Univ
期刊名称：JOURNAL OF FOOD PROTECTION
期刊影响因子：1.581
出版年份：2021
出版刊次：8
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