中文摘要：细菌代谢产物是细菌代谢的中间产物，其产生反映了细菌的代谢活性。本文报道了使用表面增强拉曼光谱(SERS)检测挥发性和非挥发性代谢物，并用该方法诊断细菌生长定量和病毒感染。首先检测并定量了细菌生长的琼脂平板上方挥发性代谢物二甲基二硫醚的时间依赖性SERS信号，该信号反映了营养物质的消耗和非挥发性代谢物的产生。代谢物积累的测定可用于细菌定量。噬菌体存在时，细菌代谢受到抑制，SERS强度相对降低，反映了病毒的初始浓度。使用多变量分析，检测病毒感染的预测准确率为93%。本文基于SERS的代谢产物监测方法为病毒感染诊断提供了新的见解。
外文摘要：Bacterial metabolites are intermediate products of bacterial metabolism and their production reflects metabolic activity. Herein, we report the use of surface-enhanced Raman spectroscopy (SERS) for detection of both volatile and nonvolatile metabolites and the application of this approach for bacterial growth quantification and diagnosis of viral infection. The time-dependent SERS signal of the volatile metabolite dimethyl disulfide in the headspace above bacteria growing on an agar plate was detected and quantified. In addition, SERS signals arising from the plate reflected nutrient consumption and production of nonvolatile metabolites. The measurement of metabolite accumulation can be used for bacterial quantification. In the presence of bacteriophage virus, bacterial metabolism is suppressed, and the relative decrease in SERS intensity reflects the initial virus concentration. Using multivariate analysis, we detect viral infection with a prediction accuracy of 93%. Our SERS-based approach for metabolite production monitoring provides new insights toward viral infection diagnosis.
外文关键词：surface-enhanced Raman spectroscopy；detection；metabolite；bacteria；virus
作者：Wang, W；Kang, SJ；Vikesland, PJ
作者单位：Virginia Tech；Sustainable Nanotechnol Ctr VTSuN
期刊名称：ENVIRONMENTAL SCIENCE & TECHNOLOGY
期刊影响因子：7.864
出版年份：2021
出版刊次：13
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