中文摘要：痤疮是青少年常见的皮肤炎症性疾病，主要与痤疮表皮杆菌的定植有关。然而，过去几年该病已扩展，约40%的成年人受到慢性感染。用抗生素治疗痤疮损伤已有几十年，但抗生素耐药性是一个日益增长的危机；因此，当务之急是寻找新的治疗靶点。研究显示，噬菌体疗法是治疗耐多药细菌感染的一种方法。本研究从健康志愿者的皮肤中成功分离到一种名为TCUCAP1的痤疮表皮杆菌噬菌体。形态学分析表明，TCUCAP1属于长尾病毒科 ，头部为二十面体，尾部不可收缩。基因组分析发现TCUCAP1由29547bp组成，G+C含量为53.83%，有56个预测开放阅读框（ORF）。ORF与噬菌体的结构、组装、宿主裂解、DNA代谢和其他功能相关。用噬菌体治疗多药耐药(MDR) 痤疮表皮杆菌诱导的皮肤炎症小鼠，可显著减少炎症病变。此外，还尝试将噬菌体制成羟乙基纤维素（HEC）乳膏，以期为人类感染提供新的抗菌制剂。可见，TCUCAP1具有诸多特性，是控制痤疮皮肤杆菌感染的理想药物。
外文摘要：Acne vulgaris, which is mostly associated with the colonization of Cutibacterium acnes (C. acnes), is a common skin inflammatory disease in teenagers. However, over the past few years, the disease has extended beyond childhood to chronically infect approximately 40% of adults. While antibiotics have been used for several decades to treat acne lesions, antibiotic resistance is a growing crisis; thus, finding a new therapeutic target is urgently needed. Studies have shown that phage therapy may be one alternative for treating multi-drug-resistant bacterial infections. In the present study, we successfully isolated a C. acnes phage named TCUCAP1 from the skin of healthy volunteers. Morphological analysis revealed that TCUCAP1 belongs to the family Siphoviridae with an icosahedral head and a non-contractile tail. Genome analysis found that TCUCAP1 is composed of 29,547 bp with a G+C content of 53.83% and 56 predicted open reading frames (ORFs). The ORFs were associated with phage structure, packing, host lysis, DNA metabolism, and additional functions. Phage treatments applied to mice with multi-drug-resistant (MDR) C.-acnes-induced skin inflammation resulted in a significant decrease in inflammatory lesions. In addition, our attempt to formulate the phage into hydroxyethyl cellulose (HEC) cream may provide new antibacterial preparations for human infections. Our results demonstrate that TCUCAP1 displays several features that make it an ideal candidate for the control of C. acnes infections.
外文关键词：Cutibacterium acnes；bacteriophages；acne vulgaris；inflammation；apoptosis；phage therapy
作者：Lam, HYP；Lai, MJ；Chen, TY；Wu, WJ；Peng, SY；Chang, KC
作者单位：Tzu Chi Univ；Buddhist Tzu Chi Gen Hosp
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
期刊影响因子：4.556
出版年份：2021
出版刊次：13
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