中文摘要：虽然在动物载脂蛋白致敏机制的某些方面已经取得了一定进展，但它们在很大程度上仍然是谜。解开这一特性可以通过研究它们与免疫系统各成分的相互作用。由于这些成分非常复杂，需要用到高通量技术。为此，本研究使用噬菌体展示随机多肽库来筛选犬过敏原Can f1；鉴定了一种与假定的γδT-细胞受体的抗原结合位点相对应的Can f1结合肽。还通过其他生化研究证实了这种相互作用。
外文摘要：Although some progress has been achieved in understanding certain aspects of the allergenic mechanism of animal lipocalins, they still remain largely enigmatic. One possibility to unravel this property is to investigate their interaction with components of the immune system. Since these components are highly complex we intended to use a high-throughput technology for this purpose. Therefore, we used phage-display of a random peptide library for panning against the dog allergen Can f 1. By this method we identified a Can f 1 binding peptide corresponding to the antigen-binding site of a putative gamma delta T-cell receptor. Additional biochemical investigations confirmed this interaction.
外文关键词：binding studies；bio-panning；high-throughput assay；immunogenicity；T-cells
作者：Habeler, M；Redl, B
作者单位：Med Univ Innsbruck
期刊名称：BIOLOGICAL CHEMISTRY
期刊影响因子：3.27
出版年份：2021
出版刊次：4
点击下载：噬菌体展示揭示载脂蛋白变应原Can f1与类似人γδT-细胞受体抗原结合区肽的相互作用