中文摘要：噬菌体疗法是抗生素药物很好的替代品，但它无法有效抵抗胃部的过酸性介质。本文提出使用聚电解质微胶囊作为保护噬菌体的手段，首先了解聚电解质对噬菌体存活的影响。研究聚阴离子和聚阳离子对大肠杆菌噬菌体活力的影响，发现聚丙烯胺在聚电解质浓度增加时会降低噬菌体的活性，而聚精氨酸则在聚电解质浓度从0.05mg/mL增加到5mg/mL时对噬菌体活性的抑制作用较低（其次是PAH）。说明聚丙烯胺对噬菌体的抑制具有静电性质，并且高离子强度阻止了PAH-蛋白衣壳复合物的形成；当聚电解质浓度从0.05mg/mL增加到1mg/mL时，聚磺苯乙烯不会影响噬菌体的活性；而当聚电解质浓度为5mg/mL时，聚磺苯乙烯降低了噬菌体的活力。葡聚糖硫酸盐在20-30%时能抑制噬菌体活性，葡聚糖在0.05至5mg/mL的较大浓度范围下抑制噬菌体活性达80%，而在5mg/mL的浓度下则失去抑制作用。
外文摘要：Phage therapy is a great alternative to antibiotic drugs, but it can't effectively overcome the over-acidic medium of the stomach. We offer the use of polyelectrolyte microcapsules as a protective means of bacteriophage. It is necessary to understand the influence of polyelectrolytes on bacteriophage survival. The work studied the effect of polyanions and polycations on the coliprotetic bacteriophage's viability. We have shown that polyallylamine decreased bacteriophage's viability during increasing polyelectrolyte concentration and polyarginine had a lower inhibitory effect (then PAH) on the activity of the bacteriophage due to polyelectrolyte concentration from 0.05 to 5 mg/mL. It was shown that the inhibition of the bacteriophage by polyallylamine had an electrostatic nature and the use of high ionic strength prevented the formation of the PAH-protein capsid complex. Polystyrene sulfonate does not affect bacteriophage viability during increasing polyelectrolyte concentration from 0.05 mg/mL to 1 mg/mL. Polystyrene sulfonate decreases the viability of bacteriophage from 5 mg/mL of polyelectrolyte concentration. Dextran sulfate inhibits bacteriophage activity at 20-30%. Dextran inhibits bacteriophage activity by 80% at diapason concentration from 0.05 to 5 mg/mL and loses the inhibition effect from a concentration of 5 mg/mL.
外文关键词：bacteriophages；polyelectrolytes；polyallylamine；polysteryne sulfonate； dextrane sulfate；polyarginine； E; coli；electrostatics
作者：Musin, EV；Kim, AL；Dubrovskii, AV；Kudryashova, EB；Ariskina, EV；Tikhonenko, SA
作者单位：Russian Acad Sci
期刊名称：POLYMERS
期刊影响因子：3.426
出版年份：2021
出版刊次：6
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