中文摘要：铜绿假单胞菌具有显著的抗生素耐药性，想要根除越来越难。用噬菌体治疗细菌感染备受推崇。本研究制备了含铜绿假单胞菌噬菌体的角膜结膜炎原位凝胶滴眼液。将囊病毒科噬菌体配制成原位凝胶制剂，它本身是一种溶液，但在接触眼睛时会变成凝胶。感染后第12天，通过组织学检查来评估原位凝胶制剂的治疗效果。研究发现滴入眼睛后，制剂粘度增加；组织学结果显示，未使用原位凝胶制剂的患者，角膜水肿、脓肿和基质结构破坏；而使用原位凝胶制剂后，出现了原位胶化和正常的角膜结构。可见，含噬菌体的原位凝胶制剂可有效治疗铜绿假单胞菌性角膜结膜炎。
外文摘要：Introduction: Eradication of Pseudomonas aeruginosa has become increasingly difficult due to its remarkable capacity to resist antibiotics. Bacteriophages have been suggested as an alternative treatment for bacterial infections.
   Methods: In-situ gel-forming eye drop containing phage against P. aeruginosa keratoconjunctivitis was prepared. The Cystoviridae phage was formulated as in-situ gel-forming formulation which is a solution formulation but turns into gel when it contacts the eye. Therapeutic effectiveness of the in-situ gel forming formulation was evaluated by histological examination on day 12 post-infection.
   Results: The viscosity of selected formulation increased when it was instilled into the eye. The histological results showed edema, abscesses, and destruction of the stromal structure of cornea in groups where no in-situ gel-forming formulation was used. In the group where in-situ gel forming formulation was used, re-epithelialization and normal corneal structure were observed.
   Conclusion: In-situ gel-forming ophthalmic formulation containing phage can be effective in the treatment of P. aeruginosa keratoconjunctivitis.
外文关键词：Bacteriophage；In-situ gel-forming system；Pseudomonas aeruginosa；Keratitis；Viscosity
作者：Rahimzadeh, G；Saeedi, M；Nokhodchi, A；Moosazadeh, M；Ghasemi, M；Rostamkalaei, SS；Mortazavi, P；Eghbali, M；Pourbakhshian, R；Rezai, MS；Hevelaee, EN
作者单位：Mazandaran Univ Med Sci；Univ Sussex；Islamic Azad Univ
期刊名称：BIOIMPACTS
期刊影响因子：3.475
出版年份：2021
出版刊次：4
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