中文摘要：饮用病原菌污染的水是水传播疾病的主要原因。为了应对这一挑战，本研究开发了一种新颖、灵敏的传感方案，即利用噬菌体T4作为生物识别元件，在光纤平台上快速检测细菌（大肠杆菌B40）。该传感方案的新颖之处在于，首先在传感表面捕获细菌（分析物）而不是噬菌体，然后传感表面通过噬菌体特异性检测细菌。在湖水基质中添加100-10(7)cfu/mL的大肠杆菌B40，传感器可轻易检测到的最低细菌浓度为1000cfu/mL。用非宿主细菌铜绿假单胞菌进行对照研究。大肠杆菌B40噬菌体T4对其宿主具有特异性，不与传感探针捕获的铜绿假单胞菌相互作用，产生的非特异性反应可忽略不计。由于噬菌体对其宿主细菌具有特异性，因此可以使用特定的噬菌体检测混合样品（包含细菌组合）中的特定细菌。传感器能够从非常高浓度（1000倍以上）的铜绿假单胞菌（非目标细菌）中检测出大肠杆菌B40（目标细菌）。
外文摘要：Consumption of water contaminated with pathogenic bacteria is a major cause of water-borne diseases. To address this challenge, we have developed a novel and sensitive sensing scheme for the rapid detection of bacteria (Escherichia coli B40) on a fiber-optic platform using bacteriophage (T4) as a bio-recognition element. The novelty of our sensing scheme is that instead of bacteriophages, bacteria (analyte) were first captured on the sensing surface and then the sensing surface was subjected to bacteriophages for specific detection of bacteria. The sensor was subjected to 100 to 10(7) cfu/mL of E. coli B40 spiked in a lake water matrix, and the least concentration of bacteria that could be easily detected was found to be 1000 cfu/mL. The control studies were performed with nonhost bacteria Pseudomonas aeruginosa. Bacteriophage T4, being specific to its host E. coli B40, did not interact with P. aeruginosa captured on the sensing probe, giving a negligible nonspecific response. Due to the specificity of bacteriophages to its host bacteria, it is possible to use this scheme to carry out the detection of specific bacteria in a mixed sample (containing a combination of bacteria) using bacteriophages specific to it. The sensor was able to detect E. coli B40 (target bacteria) even in the presence of a very high concentration (1000 times higher) of P. aeruginosa (nontarget bacteria).
外文关键词：bacteriophage；bacteria detection；fiber-optic sensor；localized surface plasmon resonance；gold nanoparticles
作者：Halkare, P；Punjabi, N；Wangchuk, J；Madugula, S；Kondabagil, K；Mukherji, S
作者单位：Indian Inst Technol
期刊名称：ACS SENSORS
期刊影响因子：7.333
出版年份：2021
出版刊次：7
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