中文摘要：猪链球菌是一种重要的人畜共患病原体。耐多药菌株的增加大大降低了传统抗生素的疗效，因此急需抗菌剂替代药物。本研究鉴定了一个重组表达的新血清型猪链球菌(Chz)原噬菌体PhiARI0460-1细胞裂解酶PlyARI。研究发现，浓度为10mug/mL的重组PlyARI对30株猪链球菌具有抗菌活性。PlyARI对猪链球菌的最低抑菌浓度低至2μg/mL，在pH4-12范围内，裂解效率可保持在较高水平。此外，在小鼠感染模型中，剂量为0.5mg的PlyARI可保护全部受到高毒力链球菌株HA9801攻击的10只小鼠。此外，通过构建绿色荧光蛋白(GFP-ARIb)评估了PlyARI的结合特异性，其中GFP与PlyAR-sh3b(细胞壁结合域，CBD)融合，显示出对猪链球菌、金黄色葡萄球菌、和马链球菌具有高亲和性，而与肺炎链球菌和无乳链球菌的亲和力中等。总的来说，PlyARI可能是一种抗菌剂药物，可用于治疗和预防链球菌感染。
外文摘要：Streptococcus suis is an important zoonotic pathogen. An increase in multi-drug-resistant strains has led to poor performance of traditional antibiotic therapies. Thus, alternative antibacterial agents are urgently needed. In this study, we identified a recombined and expressed lysin PlyARI derived from the novel serotype S. suis (Chz) prophage PhiARI0460-1. The recombinant PlyARI at a concentration of 10 mu g/mL showed high bacteriolytic activity against 30 S. suis isolates. The minimum inhibitory concentration (MIC) of PlyARI against S. suis was found to be as low as 2 mu g/mL, and the lytic efficiency could be maintained between the range of pH 4 and 12. Additionally, in a mouse infection model, a dose of 0.5 mg of PlyARI protected 10 out of 10 mice that were challenged with highly virulent S. suis strain HA9801. Furthermore, the binding specificity of PlyARI was evaluated by constructing a green fluorescent protein (GFP-ARIb), where GFP was fused with the PlyARI-SH3b (cell wall-binding domain, CBD), revealing a high affinity to S. suis, Staphylococcus aureus, and Streptococcus equi along with exhibiting a medium affinity to Streptococcus pneumoniae as well as Streptococcus agalactiae. Overall, our findings indicated that PlyARI may be an alternative antibacterial agent that was useful in treating and possibly the prevention of Streptococcal infections.
外文关键词：Streptococcus suis; PlyARI; Antibacterial agents; SH3b domain
作者：Xiao, YY；Chen, R；Li, M；Qi, ZT；Yu, YF；Pan, ZH；Yao, HC；Feng, ZX；Zhang, W
作者单位：OIE Reference Lab Swine Streptococcosis；Minist Agr；Nanjing Agr Univ；Jiangsu Acad Agr Sci
期刊名称：ARCHIVES OF MICROBIOLOGY
期刊影响因子：1.884
出版年份：2021
出版刊次：
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