中文摘要：DNA纳米技术在体外分子级设备工程领域处于领先地位，积累了大量炫丽的应用。然而，尽管DNA纳米结构在体外环境下功能强大，但在实际实施中需要克服其快速降解和随后的功能丧失。病毒是复杂的超分子组装体，能够在不利的生物环境中保护其核酸含量。受这种自然能力的启发，本研究设计了体外和体内技术，实现了MS2噬菌体病毒样颗粒（VLP）内功能性DNA纳米结构的封装和保护。研究证明ssDNA-VLPs纳米复合材料（NCs）能够以不同大小(200-1500个核苷酸(nt))、序列和结构封装单链dna(ssDNA)，并保留其功能性。此外，通过将这些NCs暴露于不利的生物条件，如人血清，证明了VLP是一种极好的保护壳。可见，这些工程NCs具有当前制造方法无法实现的关键特性。
外文摘要：DNA nanotechnology is leading the field of in vitro molecular-scale device engineering, accumulating to a dazzling array of applications. However, while DNA nanostructures' function is robust under in vitro settings, their implementation in real-world conditions requires overcoming their rapid degradation and subsequent loss of function. Viruses are sophisticated supramolecular assemblies, able to protect their nucleic acid content in inhospitable biological environments. Inspired by this natural ability, we engineered in vitro and in vivo technologies, enabling the encapsulation and protection of functional DNA nanostructures inside MS2 bacteriophage virus-like particles (VLPs). We demonstrate the ssDNA-VLPs nanocomposites' (NCs) abilities to encapsulate single-stranded-DNA (ssDNA) in a variety of sizes (200-1500 nucleotides (nt)), sequences, and structures while retaining their functionality. Moreover, by exposing these NCs to hostile biological conditions, such as human blood serum, we exhibit that the VLPs serve as an excellent protective shell. These engineered NCs pose critical properties that are yet unattainable by current fabrication methods.
外文关键词：DNA nanostructures；ssDNA encapsulation；nanocomposites；MS2 VLPs；aptamers
作者：Zilberzwige-Tal, S；Alon, DM；Gazit, D；Zachariah, S；Hollander, A；Gazit, E；Elbaz, J
作者单位：Tel Aviv Univ
期刊名称：ACS SYNTHETIC BIOLOGY
期刊影响因子：4.411
出版年份：2021
出版刊次：8
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